Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4357874..4358528 Replicon chromosome
Accession NZ_CP122454
Organism Escherichia coli strain 82.0311

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag QBY76_RS21130 Protein ID WP_000244781.1
Coordinates 4357874..4358281 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag QBY76_RS21135 Protein ID WP_000354046.1
Coordinates 4358262..4358528 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QBY76_RS21110 (4353831) 4353831..4355564 - 1734 WP_000813195.1 single-stranded-DNA-specific exonuclease RecJ -
QBY76_RS21115 (4355570) 4355570..4356280 - 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
QBY76_RS21120 (4356305) 4356305..4357201 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
QBY76_RS21125 (4357313) 4357313..4357834 + 522 WP_001055874.1 flavodoxin FldB -
QBY76_RS21130 (4357874) 4357874..4358281 - 408 WP_000244781.1 protein YgfX Toxin
QBY76_RS21135 (4358262) 4358262..4358528 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
QBY76_RS21140 (4358771) 4358771..4359751 + 981 WP_000886078.1 tRNA-modifying protein YgfZ -
QBY76_RS21145 (4359828) 4359828..4360487 - 660 WP_000250274.1 hemolysin III family protein -
QBY76_RS21150 (4360651) 4360651..4360962 - 312 WP_001182959.1 N(4)-acetylcytidine aminohydrolase -
QBY76_RS21155 (4361007) 4361007..4362440 + 1434 WP_001339296.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T276936 WP_000244781.1 NZ_CP122454:c4358281-4357874 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT276936 WP_000354046.1 NZ_CP122454:c4358528-4358262 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References