Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 4182408..4182991 Replicon chromosome
Accession NZ_CP122454
Organism Escherichia coli strain 82.0311

Toxin (Protein)


Gene name mazF Uniprot ID V0SV58
Locus tag QBY76_RS20385 Protein ID WP_000254750.1
Coordinates 4182408..4182743 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag QBY76_RS20390 Protein ID WP_000581937.1
Coordinates 4182743..4182991 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QBY76_RS20370 (4178294) 4178294..4179592 - 1299 WP_000036723.1 phosphopyruvate hydratase -
QBY76_RS20375 (4179680) 4179680..4181317 - 1638 WP_021531536.1 CTP synthase (glutamine hydrolyzing) -
QBY76_RS20380 (4181545) 4181545..4182336 - 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
QBY76_RS20385 (4182408) 4182408..4182743 - 336 WP_000254750.1 endoribonuclease MazF Toxin
QBY76_RS20390 (4182743) 4182743..4182991 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
QBY76_RS20395 (4183069) 4183069..4185303 - 2235 WP_000226815.1 GTP pyrophosphokinase -
QBY76_RS20400 (4185351) 4185351..4186652 - 1302 WP_000046817.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
QBY76_RS20405 (4186709) 4186709..4187218 + 510 Protein_3997 two-component sensor histidine kinase BarA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4187296..4188219 923


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12067.95 Da        Isoelectric Point: 8.2618

>T276935 WP_000254750.1 NZ_CP122454:c4182743-4182408 [Escherichia coli]
MVSRYVPDTGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT276935 WP_000581937.1 NZ_CP122454:c4182991-4182743 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SV58

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References