Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3629329..3629947 Replicon chromosome
Accession NZ_CP122448
Organism Escherichia coli strain 85.0412

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag QBY77_RS17590 Protein ID WP_001291435.1
Coordinates 3629729..3629947 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag QBY77_RS17585 Protein ID WP_000344800.1
Coordinates 3629329..3629703 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QBY77_RS17575 (3624419) 3624419..3625612 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
QBY77_RS17580 (3625635) 3625635..3628784 + 3150 WP_001132481.1 efflux RND transporter permease AcrB -
QBY77_RS17585 (3629329) 3629329..3629703 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
QBY77_RS17590 (3629729) 3629729..3629947 + 219 WP_001291435.1 HHA domain-containing protein Toxin
QBY77_RS17595 (3630121) 3630121..3630672 + 552 WP_000102539.1 maltose O-acetyltransferase -
QBY77_RS17600 (3630788) 3630788..3631258 + 471 WP_000136192.1 YlaC family protein -
QBY77_RS17605 (3631422) 3631422..3632972 + 1551 WP_001298569.1 cyclic-guanylate-specific phosphodiesterase PdeB -
QBY77_RS17610 (3633014) 3633014..3633367 - 354 WP_000878138.1 DUF1428 family protein -
QBY77_RS17620 (3633746) 3633746..3634057 + 312 WP_000409908.1 MGMT family protein -
QBY77_RS17625 (3634088) 3634088..3634660 - 573 WP_000779830.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T276880 WP_001291435.1 NZ_CP122448:3629729-3629947 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T276880 NZ_CP122448:3629729-3629947 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGTCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCTGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT276880 WP_000344800.1 NZ_CP122448:3629329-3629703 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT276880 NZ_CP122448:3629329-3629703 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References