Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 2590963..2592551 Replicon chromosome
Accession NZ_CP122448
Organism Escherichia coli strain 85.0412

Toxin (Protein)


Gene name hipA Uniprot ID A0A192ENQ5
Locus tag QBY77_RS12425 Protein ID WP_001125432.1
Coordinates 2591229..2592551 (+) Length 441 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID V0VAH2
Locus tag QBY77_RS12420 Protein ID WP_001304368.1
Coordinates 2590963..2591229 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QBY77_RS12405 (2587331) 2587331..2588782 + 1452 WP_000854642.1 tagaturonate reductase -
QBY77_RS12410 (2588979) 2588979..2589893 + 915 WP_001298637.1 bestrophin family protein -
QBY77_RS12415 (2589897) 2589897..2590655 - 759 WP_001286538.1 trans-aconitate 2-methyltransferase -
QBY77_RS12420 (2590963) 2590963..2591229 + 267 WP_001304368.1 type II toxin-antitoxin system antitoxin HipB Antitoxin
QBY77_RS12425 (2591229) 2591229..2592551 + 1323 WP_001125432.1 type II toxin-antitoxin system serine/threonine protein kinase toxin HipA Toxin
QBY77_RS12430 (2592727) 2592727..2593419 - 693 WP_000382466.1 hypothetical protein -
QBY77_RS12435 (2593431) 2593431..2593706 - 276 WP_000948310.1 hypothetical protein -
QBY77_RS12440 (2593708) 2593708..2593848 - 141 WP_001335097.1 hypothetical protein -
QBY77_RS12445 (2593964) 2593964..2594146 - 183 WP_001335098.1 hypothetical protein -
QBY77_RS12450 (2594316) 2594316..2595368 + 1053 WP_000568119.1 phage/plasmid replication protein, II/X family -
QBY77_RS12455 (2595380) 2595380..2595697 + 318 WP_000073834.1 DNA-binding protein -
QBY77_RS12460 (2595901) 2595901..2596143 + 243 WP_000699011.1 phage capsid protein -
QBY77_RS12465 (2596190) 2596190..2597440 + 1251 WP_033548378.1 attachment protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(4-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(14-69)


Sequences

Toxin        


Download         Length: 441 a.a.        Molecular weight: 49297.02 Da        Isoelectric Point: 8.6972

>T276877 WP_001125432.1 NZ_CP122448:2591229-2592551 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTCPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGRVRALAVKRFDRRW
NTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIAQIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGIK

Download         Length: 1323 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9957.31 Da        Isoelectric Point: 4.9632

>AT276877 WP_001304368.1 NZ_CP122448:2590963-2591229 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTSLTTFFKILQSLELSMTLCDAKNASPEA
AEQQDLEW

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A192ENQ5

Antitoxin

Source ID Structure
AlphaFold DB V0VAH2

References