Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 88849..89375 Replicon plasmid pI9455333_MCR10
Accession NZ_CP122443
Organism Enterobacter ludwigii strain I9455333cz

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag QCL67_RS26140 Protein ID WP_000323025.1
Coordinates 89088..89375 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID J5W3H0
Locus tag QCL67_RS26135 Protein ID WP_004196370.1
Coordinates 88849..89088 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QCL67_RS26100 (QCL67_26100) 84186..84378 - 193 Protein_97 MFS transporter -
QCL67_RS26105 (QCL67_26105) 84408..85385 + 978 WP_004196334.1 chromate resistance protein -
QCL67_RS26110 (QCL67_26110) 85342..86718 + 1377 Protein_99 chromate efflux transporter -
QCL67_RS26115 (QCL67_26115) 86749..87438 - 690 WP_004196322.1 hypothetical protein -
QCL67_RS26120 (QCL67_26120) 87452..88189 - 738 WP_008460272.1 HupE/UreJ family protein -
QCL67_RS26125 (QCL67_26125) 88233..88598 - 366 WP_009651956.1 hypothetical protein -
QCL67_RS26130 (QCL67_26130) 88726..88824 - 99 Protein_103 protein YdfV -
QCL67_RS26135 (QCL67_26135) 88849..89088 + 240 WP_004196370.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
QCL67_RS26140 (QCL67_26140) 89088..89375 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
QCL67_RS26145 (QCL67_26145) 89426..90460 - 1035 Protein_106 IS481 family transposase -
QCL67_RS26150 (QCL67_26150) 90532..90693 + 162 WP_023332863.1 type I toxin-antitoxin system Hok family toxin -
QCL67_RS26155 (QCL67_26155) 90788..91996 + 1209 WP_015386429.1 IS256 family transposase -
QCL67_RS26160 (QCL67_26160) 92278..92409 - 132 WP_255344245.1 hypothetical protein -
QCL67_RS26165 (QCL67_26165) 92576..92711 + 136 Protein_110 single-stranded DNA-binding protein -
QCL67_RS26170 (QCL67_26170) 92807..93628 + 822 WP_023332864.1 DUF932 domain-containing protein -
QCL67_RS26175 (QCL67_26175) 93694..94002 + 309 WP_023332865.1 DUF5983 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid mcr-10 mrkA / mrkB / mrkC / mrkF / mrkJ 1..129863 129863


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(3-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T276865 WP_000323025.1 NZ_CP122443:89088-89375 [Enterobacter ludwigii]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9018.43 Da        Isoelectric Point: 4.3894

>AT276865 WP_004196370.1 NZ_CP122443:88849-89088 [Enterobacter ludwigii]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLCNPKPVRVTLDEL

Download         Length: 240 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4QRH4

References