Detailed information of TA system TA027686

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1397341..1398257
• Replicon: chromosome
• Accession: NC_017743
• Organism: Bacillus sp. JS

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: MY9_RS07120
• Protein ID: WP_014663666.1
• Coordinates: 1397511..1398257 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: G4NV07
• Locus tag: MY9_RS07115
• Protein ID: WP_003239095.1
• Coordinates: 1397341..1397511 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MY9_RS07085	1394205..1394531	+	327	WP_014663662.1	XkdW family protein	-
MY9_RS07090	1394528..1394692	+	165	WP_014663663.1	XkdX family protein	-
MY9_RS07095	1394737..1395576	+	840	WP_014663664.1	phage-like element PBSX protein XepA	-
MY9_RS07100	1395629..1395898	+	270	WP_003232655.1	hemolysin XhlA family protein	-
MY9_RS07105	1395911..1396174	+	264	WP_003232653.1	phage holin	-
MY9_RS07110	1396187..1397080	+	894	WP_014663665.1	N-acetylmuramoyl-L-alanine amidase	-
MY9_RS21530	1397118..1397255	-	138	WP_136654612.1	three component toxin-antitoxin-antitoxin system antitoxin SpoIISC	-
MY9_RS07115	1397341..1397511	-	171	WP_003239095.1	three component toxin-antitoxin-antitoxin system antitoxin SpoIISB	Antitoxin
MY9_RS07120	1397511..1398257	-	747	WP_014663666.1	toxin-antitoxin-antitoxin system toxin SpoIISA	Toxin
MY9_RS07125	1398367..1399368	-	1002	WP_014663667.1	inorganic phosphate transporter family protein	-
MY9_RS07130	1399381..1399998	-	618	WP_003218470.1	DUF47 domain-containing protein	-
MY9_RS07135	1400275..1401591	-	1317	WP_014663668.1	serine/threonine exchanger	-
MY9_RS07140	1401979..1402929	+	951	WP_014663669.1	ring-cleaving dioxygenase	-
MY9_RS21535	1403014..1403158	+	145	Protein_1343	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-238)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29084.59 Da
• Isoelectric Point: 4.6145

>T27686 WP_014663666.1 NC_017743:c1398257-1397511 [Bacillus sp. JS]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWDRYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CQYTTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKMVLIPFTIETKNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Nucleotide

• Length: 747 bp

>T27686 NC_017743:c1398257-1397511 [Bacillus sp. JS]
ATGGTTTTATTCTTTCAGATCATGGTCTGGTGCATTGTGGCTGGACTGGGGTTATACGTGTATGCCACGTGGCGTTTTGA
AGCGAAGGTCAAAGAAAAAATGTCCGCCATCCGGAAAACATGGTATTTGCTGTTTGTTCTGGGCGCTATGGTATACTGGA
CATATGAGCCCACTTCCCTATTTACCCACTGGGATCGGTATCTCATTGTCGCAGTCAGCTTTGCTTTGATTGATGCTTTC
ATCTTCTTAAGTGCATACGTCAAAAAACTGGCTGGCAGCGAGCTTGAAACAGACACAAGAGAAATTCTTGAAGAAAACAA
CGAAATGCTCCACATGTATCTCAATCGGCTGAAAACATACCAATACCTATTGAAAAACGAACCGATCCATGTTTATTATG
GAAGTATAGATGCTTATGCTGAAGGCATTGATAAGCTGCTGAAAACCTATGCTGATAAAATGAACTTAACGGCTTCTCTT
TGCCAATATACGACGCAGGCTGATAAAGACCGGTTAACCGAGCATATGGATGATCCGGCAGATGTACAAACACGGCTCGA
TCGAAAGGATGTTTATTACGACCAATACGGAAAAATGGTTCTCATCCCTTTTACCATTGAGACAAAGAACTATGTCATCA
AGCTGACGTCTGACAGCATTGTTACGGAATTCGATTATTTGCTATTTACGTCATTAACAAGCATATATGATTTGGTGCTG
CCAATTGAGGAGGAAGGTGAAGGATAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6555.59 Da
• Isoelectric Point: 10.8401

>AT27686 WP_003239095.1 NC_017743:c1397511-1397341 [Bacillus sp. JS]
MERAFQNGCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Nucleotide

• Length: 171 bp

>AT27686 NC_017743:c1397511-1397341 [Bacillus sp. JS]
ATGGAACGTGCGTTTCAAAACGGATGCGAGCCCAGAGCGGCCAAACCGTTTAAAATCCTGAAAAAACGTTCAACCACCAG
TGTCGCAAGCTATCAAGTCAGTCCGCACACAGCAAGAATCTTCAAAGAAAACGAACGGCTGATTGACGAGTATAAACGAA
AAAAAGCATGA

Structures

Antitoxin

• AlphaFold DB: G4NV07