Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 515490..516126 Replicon chromosome
Accession NC_017743
Organism Bacillus sp. JS

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag MY9_RS02625 Protein ID WP_003156187.1
Coordinates 515776..516126 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag MY9_RS02620 Protein ID WP_003225183.1
Coordinates 515490..515771 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MY9_RS02600 511850..512449 - 600 WP_014662850.1 rhomboid family intramembrane serine protease -
MY9_RS02605 512543..512908 + 366 WP_014475874.1 holo-ACP synthase -
MY9_RS02610 513074..514090 + 1017 WP_080478309.1 outer membrane lipoprotein carrier protein LolA -
MY9_RS02615 514205..515374 + 1170 WP_014662852.1 alanine racemase -
MY9_RS02620 515490..515771 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
MY9_RS02625 515776..516126 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
MY9_RS02630 516241..517065 + 825 WP_014662853.1 RsbT co-antagonist protein RsbRA -
MY9_RS02635 517070..517435 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
MY9_RS02640 517439..517840 + 402 WP_003240352.1 serine/threonine-protein kinase RsbT -
MY9_RS02645 517852..518859 + 1008 WP_014662854.1 phosphoserine phosphatase RsbU -
MY9_RS02650 518921..519250 + 330 WP_014662855.1 anti-sigma factor antagonist RsbV -
MY9_RS02655 519247..519729 + 483 WP_014662856.1 anti-sigma B factor RsbW -
MY9_RS02660 519695..520483 + 789 WP_014662857.1 RNA polymerase sigma factor SigB -
MY9_RS02665 520483..521082 + 600 WP_014662858.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T27685 WP_003156187.1 NC_017743:515776-516126 [Bacillus sp. JS]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T27685 NC_017743:515776-516126 [Bacillus sp. JS]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT27685 WP_003225183.1 NC_017743:515490-515771 [Bacillus sp. JS]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT27685 NC_017743:515490-515771 [Bacillus sp. JS]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References