Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5315214..5315839 Replicon chromosome
Accession NZ_CP122389
Organism Klebsiella pneumoniae strain KP9650

Toxin (Protein)


Gene name vapC Uniprot ID A0A483GDF6
Locus tag QCB43_RS25945 Protein ID WP_004187928.1
Coordinates 5315214..5315597 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag QCB43_RS25950 Protein ID WP_004150355.1
Coordinates 5315597..5315839 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QCB43_RS25930 (QCB43_25930) 5312580..5313482 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
QCB43_RS25935 (QCB43_25935) 5313479..5314114 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
QCB43_RS25940 (QCB43_25940) 5314111..5315040 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
QCB43_RS25945 (QCB43_25945) 5315214..5315597 - 384 WP_004187928.1 type II toxin-antitoxin system VapC family toxin Toxin
QCB43_RS25950 (QCB43_25950) 5315597..5315839 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
QCB43_RS25955 (QCB43_25955) 5316033..5316950 + 918 WP_004187929.1 alpha/beta hydrolase -
QCB43_RS25960 (QCB43_25960) 5316964..5317905 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
QCB43_RS25965 (QCB43_25965) 5317950..5318387 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
QCB43_RS25970 (QCB43_25970) 5318384..5319244 - 861 WP_004146232.1 virulence factor BrkB family protein -
QCB43_RS25975 (QCB43_25975) 5319238..5319837 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-110)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14407.64 Da        Isoelectric Point: 7.3178

>T276827 WP_004187928.1 NZ_CP122389:c5315597-5315214 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFTGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT276827 WP_004150355.1 NZ_CP122389:c5315839-5315597 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A483GDF6

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References