Detailed information of TA system TA027676

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YafQ(toxin)
• Location: 1793..2423
• Replicon: plasmid pHCW
• Accession: NC_017738
• Organism: Helicobacter cetorum MIT 00-7128

Toxin (Protein)

• Gene name: relE
• Uniprot ID: I0EQ05
• Locus tag: HCW_RS08930
• Protein ID: WP_014661884.1
• Coordinates: 2157..2423 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: I0EQ04
• Locus tag: HCW_RS08925
• Protein ID: WP_014661883.1
• Coordinates: 1793..2176 (+)
• Length: 128 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HCW_RS09190	174..488	+	315	WP_155802334.1	RepA	-
HCW_RS09195	376..1581	+	1206	WP_196794384.1	replication initiation protein	-
HCW_RS08925	1793..2176	+	384	WP_014661883.1	hypothetical protein	Antitoxin
HCW_RS08930	2157..2423	+	267	WP_014661884.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
HCW_RS09615	2423..3982	+	1560	WP_014661885.1	hypothetical protein	-
HCW_RS08945	4534..4800	+	267	WP_014661887.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	-
HCW_RS08950	4821..6041	-	1221	WP_043902860.1	replication initiation protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..12465	12465

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (2-88)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10450.39 Da
• Isoelectric Point: 9.5126

>T27676 WP_014661884.1 NC_017738:2157-2423 [Helicobacter cetorum MIT 00-7128]
VLKLILKKSFQKDFDKLLLNSFDDSVLNKVILSLRKKEPLEPQFKDHELKGKWKPYRECHIKTDILLVYLVKDDELILVR
LGSHSELF

Nucleotide

• Length: 267 bp

>T27676 NC_017738:2157-2423 [Helicobacter cetorum MIT 00-7128]
GTGTTGAAACTAATTCTTAAAAAATCTTTTCAAAAAGATTTTGACAAATTACTTTTGAATAGTTTTGATGATAGCGTTTT
GAATAAAGTTATTCTATCCTTACGAAAAAAAGAACCACTAGAACCACAATTTAAAGACCATGAGCTAAAGGGGAAATGGA
AACCCTACAGAGAATGCCATATAAAAACCGACATTCTACTTGTCTATCTAGTGAAAGATGATGAGCTTATTTTAGTAAGG
CTAGGTAGTCATAGCGAATTGTTTTAA

Antitoxin

Protein

• Length: 128 a.a.
• Molecular weight: 14951.68 Da
• Isoelectric Point: 6.4772

>AT27676 WP_014661883.1 NC_017738:1793-2176 [Helicobacter cetorum MIT 00-7128]
MANTSTQKDYSHLSKRQLFNLMDKTEQKIKKIQNDRIAFKEKMDKELEKQNRNFNNKLDELTKLLQEINLAFDSKRANDC
CFGHETPNLETQQAMRDIDNKETSLEVDDFSNYSNERKRALGVETNS

Nucleotide

• Length: 384 bp

>AT27676 NC_017738:1793-2176 [Helicobacter cetorum MIT 00-7128]
ATGGCTAATACTTCTACACAAAAAGACTACTCACATTTAAGCAAGAGACAGCTTTTTAATTTAATGGATAAGACAGAACA
AAAAATTAAAAAGATACAGAACGACAGAATAGCCTTTAAAGAAAAAATGGATAAAGAGCTAGAAAAACAAAACAGAAATT
TTAATAACAAACTAGATGAGCTAACCAAACTCTTACAAGAAATAAATCTCGCCTTTGATAGTAAAAGAGCTAATGATTGT
TGCTTTGGACATGAGACCCCAAACCTTGAAACCCAACAAGCCATGCGAGATATAGACAACAAAGAAACAAGTTTAGAAGT
TGATGATTTTTCTAATTACAGCAACGAAAGAAAAAGGGCTTTAGGTGTTGAAACTAATTCTTAA

Structures

Toxin

• AlphaFold DB: I0EQ05

Antitoxin

• AlphaFold DB: I0EQ04