Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 2151979..2152476 Replicon chromosome
Accession NZ_CP122319
Organism Escherichia coli strain BW25113

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag QCG47_RS10235 Protein ID WP_001173343.1
Coordinates 2152303..2152476 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag QCG47_RS10230 Protein ID WP_000398619.1
Coordinates 2151979..2152275 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QCG47_RS10210 (2148710) 2148710..2149429 - 720 WP_000611288.1 two-component system response regulator DcuR -
QCG47_RS10215 (2149426) 2149426..2151057 - 1632 WP_001216477.1 sensor histidine kinase -
QCG47_RS10220 (2151238) 2151238..2151468 + 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
QCG47_RS10225 (2151480) 2151480..2151752 + 273 WP_000405651.1 GNAT family N-acetyltransferase -
QCG47_RS10230 (2151979) 2151979..2152275 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
QCG47_RS10235 (2152303) 2152303..2152476 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
QCG47_RS10240 (2152595) 2152595..2154112 - 1518 WP_001295074.1 lysine--tRNA ligase -
QCG47_RS10245 (2154349) 2154349..2155806 - 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T276755 WP_001173343.1 NZ_CP122319:2152303-2152476 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT276755 WP_000398619.1 NZ_CP122319:2151979-2152275 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References