Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 241653..242295 Replicon chromosome
Accession NC_017729
Organism Bacillus anthracis str. H9401 (Bacillus anthracis NCCP 12889)

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag H9401_RS01375 Protein ID WP_000635963.1
Coordinates 241945..242295 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag H9401_RS01370 Protein ID WP_000004570.1
Coordinates 241653..241940 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9401_RS01345 236969..237931 + 963 Protein_232 UV DNA damage repair endonuclease UvsE -
H9401_RS01350 237924..238496 - 573 WP_000906917.1 rhomboid family intramembrane serine protease -
H9401_RS01355 238589..238948 + 360 WP_000635040.1 holo-ACP synthase -
H9401_RS01360 239105..240055 + 951 WP_025388382.1 outer membrane lipoprotein carrier protein LolA -
H9401_RS01365 240174..241343 + 1170 WP_000390596.1 alanine racemase -
H9401_RS01370 241653..241940 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
H9401_RS01375 241945..242295 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
H9401_RS01380 242363..244531 + 2169 WP_000426225.1 RNA-binding transcriptional accessory protein -
H9401_RS01385 244589..244705 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
H9401_RS01390 244901..245359 + 459 WP_000344248.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T27672 WP_000635963.1 NC_017729:241945-242295 [Bacillus anthracis str. H9401]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T27672 NC_017729:241945-242295 [Bacillus anthracis str. H9401]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGTGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT27672 WP_000004570.1 NC_017729:241653-241940 [Bacillus anthracis str. H9401]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT27672 NC_017729:241653-241940 [Bacillus anthracis str. H9401]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References