Detailed information of TA system TA027671

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 66415..66974
• Replicon: plasmid p948
• Accession: NC_017724
• Organism: Escherichia coli ETEC H10407

Toxin (Protein)

• Gene name: relE
• Uniprot ID: E3PPG0
• Locus tag: ETEC_RS26890
• Protein ID: WP_000421265.1
• Coordinates: 66415..66690 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: E3PPG1
• Locus tag: ETEC_RS26895
• Protein ID: WP_001743519.1
• Coordinates: 66690..66974 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ETEC_RS30925	61563..61637	+	75	Protein_62	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
ETEC_RS31205	61635..61949	-	315	Protein_63	hypothetical protein	-
ETEC_RS30930	62168..62383	-	216	Protein_64	hypothetical protein	-
ETEC_RS30935	62454..62711	+	258	Protein_65	transposase	-
ETEC_RS26870	62779..63930	+	1152	WP_001254932.1	IS30-like element IS30 family transposase	-
ETEC_RS30940	63940..64824	+	885	Protein_67	IS3-like element ISEc52 family transposase	-
ETEC_RS26885	65176..66252	-	1077	WP_000610005.1	hypothetical protein	-
ETEC_RS26890	66415..66690	-	276	WP_000421265.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ETEC_RS26895	66690..66974	-	285	WP_001743519.1	ribbon-helix-helix domain-containing protein	Antitoxin
ETEC_RS30945	67728..69448	-	1721	Protein_71	ATP-binding protein	-
ETEC_RS26910	69441..70138	-	698	Protein_72	IS1 family transposase	-
ETEC_RS26915	70587..70784	-	198	WP_001381161.1	hypothetical protein	-
ETEC_RS26920	70997..71563	-	567	WP_000904908.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	etpA / etpB / cfaA / cfaB / cfaC / cfaE / cfaD'	1..94797	94797
	-	inside	IScluster/Tn	-	-	62454..74742	12288

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10742.45 Da
• Isoelectric Point: 9.9324

>T27671 WP_000421265.1 NC_017724:c66690-66415 [Escherichia coli ETEC H10407]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQASVILLTHPRMGEQLFQFELREVRRIFAGEYEIRYELTGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T27671 NC_017724:c66690-66415 [Escherichia coli ETEC H10407]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
GGCCGCCAGAACGGTGCAGTCCCTGACACAGGCATCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACTCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTACTGGCCAGACTATTTAT
GTATTGCGTCTGTGGCATACGCGGGAAAACAGATAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10490.95 Da
• Isoelectric Point: 5.6546

>AT27671 WP_001743519.1 NC_017724:c66974-66690 [Escherichia coli ETEC H10407]
MQMKNNCAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQIIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT27671 NC_017724:c66974-66690 [Escherichia coli ETEC H10407]
ATGCAAATGAAAAACAATTGTGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGGTTATCAAGCAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTAACATCCGGACAGATTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: E3PPG0

Antitoxin

• AlphaFold DB: E3PPG1