Detailed information of TA system TA027670

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 22647..23125
• Replicon: plasmid p948
• Accession: NC_017724
• Organism: Escherichia coli ETEC H10407

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: ETEC_RS26610
• Protein ID: WP_000969994.1
• Coordinates: 22647..22859 (-)
• Length: 71 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: E3PPA5
• Locus tag: ETEC_RS26615
• Protein ID: WP_000079921.1
• Coordinates: 22856..23125 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ETEC_RS30900	19552..19665	-	114	Protein_12	hypothetical protein	-
ETEC_RS26580	19754..20119	+	366	WP_000124098.1	hypothetical protein	-
ETEC_RS26585	20119..20552	+	434	Protein_14	transposase zinc-binding domain-containing protein	-
ETEC_RS26600	21929..22231	-	303	Protein_16	transposase	-
ETEC_RS29475	22263..22466	+	204	Protein_17	IS91 family transposase	-
ETEC_RS26610	22647..22859	-	213	WP_000969994.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ETEC_RS26615	22856..23125	-	270	WP_000079921.1	hypothetical protein	Antitoxin
ETEC_RS26620	24030..24887	-	858	WP_000130956.1	incFII family plasmid replication initiator RepA	-
ETEC_RS29490	24880..24954	-	75	WP_001365705.1	RepA leader peptide Tap	-
ETEC_RS26630	25180..25440	-	261	WP_000083816.1	replication regulatory protein RepA	-
ETEC_RS29500	25724..25855	-	132	WP_064765989.1	type I toxin-antitoxin system Hok family toxin	-
ETEC_RS26640	25893..27106	+	1214	WP_173353254.1	IS3 family transposase	-
ETEC_RS26645	27149..27775	+	627	Protein_25	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	etpA / etpB / cfaA / cfaB / cfaC / cfaE / cfaD'	1..94797	94797
	-	inside	IScluster/Tn	-	-	20119..36979	16860

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-66)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 71 a.a.
• Molecular weight: 8175.38 Da
• Isoelectric Point: 8.5821

>T27670 WP_000969994.1 NC_017724:c22859-22647 [Escherichia coli ETEC H10407]
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVHR

Nucleotide

• Length: 213 bp

>T27670 NC_017724:c22859-22647 [Escherichia coli ETEC H10407]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
GGCCGCCATTGAACTGGACGAACGAATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCATCGATAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10224.63 Da
• Isoelectric Point: 9.2950

>AT27670 WP_000079921.1 NC_017724:c23125-22856 [Escherichia coli ETEC H10407]
MAQVNMSLRIDAELKDAFMAAAKNMDRNGSQLIRDFMRQTVERQHNTWFRNQVEAGRQQLKCGDVLPHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT27670 NC_017724:c23125-22856 [Escherichia coli ETEC H10407]
ATGGCACAGGTTAATATGAGTTTAAGAATTGACGCTGAACTGAAAGATGCTTTTATGGCCGCTGCAAAAAACATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGGCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTAACCAGGTTG
AGGCAGGACGTCAGCAACTCAAGTGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA

Structures

Antitoxin

• AlphaFold DB: E3PPA5