Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) rnlAB/RnlA-RnlB
Location 1296531..1297968 Replicon chromosome
Accession NZ_CP122317
Organism Escherichia coli strain HT-948

Toxin (Protein)


Gene name rnlA Uniprot ID P52129
Locus tag QCG49_RS06310 Protein ID WP_000155570.1
Coordinates 1296531..1297604 (+) Length 358 a.a.

Antitoxin (Protein)


Gene name rnlB Uniprot ID P52130
Locus tag QCG49_RS06315 Protein ID WP_000461704.1
Coordinates 1297597..1297968 (+) Length 124 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QCG49_RS06295 (1291964) 1291964..1294153 - 2190 WP_000136402.1 DEAD/DEAH box helicase -
QCG49_RS06300 (1294150) 1294150..1295766 - 1617 WP_000445380.1 anti-bacteriophage protein AbpA -
QCG49_RS06305 (1296126) 1296126..1296389 - 264 WP_000502050.1 DUF1524 domain-containing protein -
QCG49_RS06310 (1296531) 1296531..1297604 + 1074 WP_000155570.1 type II toxin-antitoxin system toxin mRNA endoribonuclease RnlA Toxin
QCG49_RS06315 (1297597) 1297597..1297968 + 372 WP_000461704.1 type II toxin-antitoxin system antitoxin RnlB Antitoxin
QCG49_RS06320 (1298323) 1298323..1299186 + 864 WP_000787597.1 GTPase family protein -
QCG49_RS06325 (1299278) 1299278..1300099 + 822 WP_000197391.1 DUF932 domain-containing protein -
QCG49_RS06330 (1300316) 1300316..1301017 + 702 WP_000189409.1 WYL domain-containing protein -
QCG49_RS06335 (1301058) 1301058..1301294 + 237 WP_001144029.1 protein YpjK -
QCG49_RS06340 (1301294) 1301294..1301737 + 444 WP_000824222.1 lipoprotein YfjS -
QCG49_RS06345 (1301761) 1301761..1302228 + 468 WP_000702495.1 protein YfjT -
QCG49_RS06350 (1302615) 1302615..1302767 - 153 Protein_1245 arsenate reductase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.



Sequences


Toxin        


Download         Length: 358 a.a.        Molecular weight: 40061.13 Da        Isoelectric Point: 6.8293

>T276695 WP_000155570.1 NZ_CP122317:1296531-1297604 [Escherichia coli]
MTIRSYKNLNLVRANIETESRQFIENKNYSIQSIGPMPGSRAGLRVVFTRPGVNLATVDIFYNGDGSTTIQYLTGANRSL
GQELADHLFETINPAEFEQVNMVLQGFVETSVLPVLELSADESHIEFREHSRNAHTVVWKIISTSYQDELTVSLHITTGK
LQIQGRPLSCYRVFTFNLAALLDLQGLEKVLIRQEDGKANIVQQEVARTYLQTVMADAYPHLHVTAEKLLVSGLCVKLAA
PDLPDYCMLLYPELRTIEGVLKSKMSGLGMPVQQPAGFGTYFDKPAAHYILKPQFAATLRPEQINIISTAYTFFNVERHS
LFHMETVVDASRMISDMARLMGKATRAWGIIKDLYIV

Download         Length: 1074 bp


Antitoxin


Download         Length: 124 a.a.        Molecular weight: 13664.69 Da        Isoelectric Point: 4.2391

>AT276695 WP_000461704.1 NZ_CP122317:1297597-1297968 [Escherichia coli]
MFEITGINVSGALKAVVMATGFENPLSSVNEIETKLSALLGSETTGEILFDLLCANGPEWNRFVTLEMKYGRIMLDTAKI
IDEQDVPTHILSKLTFTLRNHPEYLEASVLSPDDVRQVLSMDF

Download         Length: 372 bp


Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 6Y2R
PDB 7AEX
PDB 6Y2Q
PDB 4I8O
PDB 6Y2P
PDB 6Y2S


Antitoxin

Source ID Structure
PDB 6Y2P
AlphaFold DB A0A373F4G4

References