Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4447221..4447875 Replicon chromosome
Accession NZ_CP122316
Organism Escherichia coli strain HT-946

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag QCG48_RS21825 Protein ID WP_000244777.1
Coordinates 4447468..4447875 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag QCG48_RS21820 Protein ID WP_000354046.1
Coordinates 4447221..4447487 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QCG48_RS21795 (4442390) 4442390..4443133 + 744 WP_000951964.1 SDR family oxidoreductase -
QCG48_RS21800 (4443190) 4443190..4444623 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
QCG48_RS21805 (4444668) 4444668..4444979 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
QCG48_RS21810 (4445143) 4445143..4445802 + 660 WP_000250274.1 hemolysin III family protein -
QCG48_RS21815 (4445998) 4445998..4446978 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
QCG48_RS21820 (4447221) 4447221..4447487 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
QCG48_RS21825 (4447468) 4447468..4447875 + 408 WP_000244777.1 protein YgfX Toxin
QCG48_RS21830 (4447915) 4447915..4448436 - 522 WP_001055874.1 flavodoxin FldB -
QCG48_RS21835 (4448548) 4448548..4449444 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
QCG48_RS21840 (4449469) 4449469..4450179 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
QCG48_RS21845 (4450185) 4450185..4451918 + 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T276684 WP_000244777.1 NZ_CP122316:4447468-4447875 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT276684 WP_000354046.1 NZ_CP122316:4447221-4447487 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References