Detailed information of TA system TA027664

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 1694438..1695480
• Replicon: chromosome
• Accession: NC_017671
• Organism: Stenotrophomonas maltophilia D457

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: SMD_RS07975
• Protein ID: WP_003109777.1
• Coordinates: 1694905..1695480 (+)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: I3TV68
• Locus tag: SMD_RS07970
• Protein ID: WP_003050245.1
• Coordinates: 1694438..1694908 (+)
• Length: 157 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SMD_RS07935	1689829..1691248	-	1420	Protein_1530	TIGR03752 family integrating conjugative element protein	-
SMD_RS07940	1691238..1692149	-	912	WP_003105643.1	TIGR03749 family integrating conjugative element protein	-
SMD_RS07945	1692146..1692838	-	693	WP_003105641.1	TIGR03746 family integrating conjugative element protein	-
SMD_RS07950	1692835..1693233	-	399	WP_003105639.1	TIGR03750 family conjugal transfer protein	-
SMD_RS07955	1693245..1693604	-	360	WP_003090173.1	TIGR03745 family integrating conjugative element membrane protein	-
SMD_RS07960	1693621..1693854	-	234	WP_003050225.1	TIGR03758 family integrating conjugative element protein	-
SMD_RS07965	1693851..1694234	-	384	WP_003120001.1	RAQPRD family integrative conjugative element protein	-
SMD_RS07970	1694438..1694908	+	471	WP_003050245.1	helix-turn-helix domain-containing protein	Antitoxin
SMD_RS07975	1694905..1695480	+	576	WP_003109777.1	PIN domain-containing protein	Toxin
SMD_RS07980	1695498..1696412	+	915	WP_003105629.1	AAA family ATPase	-
SMD_RS07985	1696409..1696879	+	471	WP_003105626.1	hypothetical protein	-
SMD_RS07990	1696876..1697376	+	501	WP_003090159.1	hypothetical protein	-
SMD_RS07995	1697376..1698278	+	903	WP_003105624.1	nucleotidyltransferase domain-containing protein	-
SMD_RS08000	1698317..1699042	+	726	WP_003050273.1	CBASS effector endonuclease NucC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1645653..1745007	99354

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-114)

Antitoxin

• Helix-turn-helix domain, group 17 (85-133)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21644.79 Da
• Isoelectric Point: 5.6172

>T27664 WP_003109777.1 NC_017671:1694905-1695480 [Stenotrophomonas maltophilia D457]
MRHSPFTAVYDACVLYPAPLRDFLMWLGLSGRFRARWSQAIHEEWKRNLLINRPDLTRVQVDRTSDLMDRAIPDGLVEGY
EALVAGLTLPDPDDRHVLAAAIRCGASVIVTFNERDFPNDLLAPYGIESQHPDEFVDNLLDLDAAAVVSAAQRQRAQLKH
PPIEVDRYLEILLRQGLVQTTKVLATYRTIL

Nucleotide

• Length: 576 bp

>T27664 NC_017671:1694905-1695480 [Stenotrophomonas maltophilia D457]
ATGAGGCATTCCCCATTCACCGCCGTCTACGACGCGTGCGTTCTATATCCCGCGCCACTGCGGGATTTCCTGATGTGGCT
CGGCCTGTCTGGCCGCTTCCGGGCGCGGTGGAGCCAAGCCATTCATGAGGAGTGGAAACGCAACCTGTTGATCAACCGCC
CCGATCTCACCCGGGTTCAGGTCGACAGGACGTCGGATCTCATGGACCGGGCAATTCCGGACGGCTTGGTGGAGGGCTAC
GAAGCGCTCGTGGCAGGCCTGACATTGCCCGATCCGGACGACCGGCACGTCCTGGCTGCGGCGATTCGCTGCGGTGCGAG
CGTGATTGTGACGTTCAACGAACGGGATTTCCCGAACGATCTGCTGGCTCCGTACGGCATCGAATCGCAGCACCCCGATG
AGTTTGTGGACAACCTACTGGATCTGGACGCGGCCGCCGTGGTGTCGGCTGCGCAGCGCCAGCGTGCCCAACTCAAGCAT
CCGCCGATCGAGGTGGACCGCTATCTCGAAATCCTGCTGCGCCAGGGCCTTGTGCAAACGACCAAGGTGCTGGCGACCTA
TCGCACCATTCTCTGA

Antitoxin

Protein

• Length: 157 a.a.
• Molecular weight: 17245.62 Da
• Isoelectric Point: 6.3803

>AT27664 WP_003050245.1 NC_017671:1694438-1694908 [Stenotrophomonas maltophilia D457]
MTTATAQSKMTLPAAGEVKAAVQGQRALAAYLATQFETQHIQIFDDHKQAHQVELPTSALRLLVDILAELADGNAVKVVP
VHAELTTQEAADLLNVSRPHFVKLLEDGVLAFHRTGKHRRVRFADLMQYKEARERASEQAMAELAQQSQELGMGYE

Nucleotide

• Length: 471 bp

>AT27664 NC_017671:1694438-1694908 [Stenotrophomonas maltophilia D457]
ATGACCACCGCCACTGCCCAATCCAAGATGACCCTTCCCGCCGCGGGAGAGGTGAAGGCAGCCGTCCAGGGTCAACGTGC
CTTGGCGGCTTACCTCGCAACCCAGTTCGAGACGCAGCACATCCAGATCTTTGATGACCACAAACAGGCTCATCAGGTGG
AACTGCCTACCTCGGCTTTACGCCTGCTGGTCGACATCCTGGCCGAGTTGGCCGATGGCAATGCAGTAAAGGTGGTGCCC
GTCCATGCAGAGCTGACGACCCAAGAGGCGGCGGACTTGCTCAACGTGTCCCGTCCCCATTTCGTCAAGCTGCTCGAAGA
TGGGGTGTTGGCATTTCATCGCACCGGCAAGCACCGCAGGGTGAGGTTCGCCGATCTGATGCAGTACAAGGAAGCGCGCG
AGCGCGCCAGTGAGCAGGCGATGGCCGAACTCGCTCAGCAGTCGCAAGAGTTGGGAATGGGATACGAATGA

Structures

Antitoxin

• AlphaFold DB: I3TV68