276633

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yafW-ykfI/CbtA-CbeA
Location 2060199..2060878 Replicon chromosome
Accession NZ_CP122315
Organism Escherichia coli strain HT-947

Toxin (Protein)


Gene name ykfI Uniprot ID P77692
Locus tag QCG46_RS09990 Protein ID WP_000854672.1
Coordinates 2060537..2060878 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name yafW Uniprot ID Q47684
Locus tag QCG46_RS09985 Protein ID WP_000070395.1
Coordinates 2060199..2060516 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QCG46_RS09935 (2055246) 2055246..2056109 + 864 WP_001065553.1 GTPase family protein -
QCG46_RS09940 (2056201) 2056201..2057022 + 822 WP_000197389.1 DUF932 domain-containing protein -
QCG46_RS09945 (2057239) 2057239..2057430 + 192 Protein_1931 DeoR family transcriptional regulator -
QCG46_RS09950 (2057426) 2057426..2057653 + 228 WP_001548158.1 protein YpjK -
QCG46_RS09955 (2057653) 2057653..2058096 + 444 WP_000824223.1 lipoprotein YafY -
QCG46_RS09960 (2058119) 2058119..2058586 + 468 WP_001547765.1 protein YkfB -
QCG46_RS09965 (2058663) 2058663..2058902 + 240 WP_000194654.1 DUF905 family protein -
QCG46_RS09970 (2059000) 2059000..2059458 + 459 WP_000211838.1 antirestriction protein -
QCG46_RS09975 (2059474) 2059474..2059950 + 477 WP_000811693.1 RadC family protein -
QCG46_RS09980 (2059959) 2059959..2060180 + 222 WP_000691994.1 DUF987 domain-containing protein -
QCG46_RS09985 (2060199) 2060199..2060516 + 318 WP_000070395.1 type IV toxin-antitoxin system antitoxin YafW Antitoxin
QCG46_RS09990 (2060537) 2060537..2060878 + 342 WP_000854672.1 type IV toxin-antitoxin system toxin YkfI Toxin
QCG46_RS10000 (2061450) 2061450..2062703 - 1254 WP_000893278.1 glutamate-5-semialdehyde dehydrogenase -
QCG46_RS10005 (2062715) 2062715..2063818 - 1104 WP_001285288.1 glutamate 5-kinase -
QCG46_RS10010 (2064106) 2064106..2065161 + 1056 WP_000749863.1 phosphoporin PhoE -
QCG46_RS10015 (2065200) 2065200..2065601 - 402 WP_000174689.1 sigma factor-binding protein Crl -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(6-104)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12905.01 Da        Isoelectric Point: 9.6543

>T276633 WP_000854672.1 NZ_CP122315:2060537-2060878 [Escherichia coli]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Download         Length: 342 bp

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11939.59 Da        Isoelectric Point: 6.7391

>AT276633 WP_000070395.1 NZ_CP122315:2060199-2060516 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Download         Length: 318 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB P77692

Antitoxin

Source ID Structure
AlphaFold DB Q47684

References