Detailed information of TA system TA027660

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yeeU/CbtA-CbeA
• Location: 4770553..4771260
• Replicon: chromosome
• Accession: NC_017664
• Organism: Escherichia coli W

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: E0J2C8
• Locus tag: WFL_RS23760
• Protein ID: WP_000691790.1
• Coordinates: 4770919..4771260 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: WFL_RS23755
• Protein ID: WP_000939438.1
• Coordinates: 4770553..4770888 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WFL_RS23730	4766500..4766718	-	219	WP_001064742.1	AlpA family phage regulatory protein	-
WFL_RS23735	4766836..4767450	-	615	WP_000772909.1	inovirus Gp2 family protein	-
WFL_RS23740	4768043..4768996	+	954	WP_000290405.1	hypothetical protein	-
WFL_RS23745	4769232..4770050	+	819	WP_000761991.1	DUF945 domain-containing protein	-
WFL_RS23750	4770080..4770553	+	474	WP_001292742.1	DNA repair protein RadC	-
WFL_RS23755	4770553..4770888	+	336	WP_000939438.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
WFL_RS23760	4770919..4771260	+	342	WP_000691790.1	TA system toxin CbtA family protein	Toxin
WFL_RS23765	4771375..4772208	+	834	WP_001192746.1	DUF4942 domain-containing protein	-
WFL_RS23770	4772278..4772673	+	396	WP_000152741.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	-
WFL_RS23775	4772666..4773613	+	948	WP_000342500.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
WFL_RS23780	4774006..4775271	+	1266	WP_001218329.1	integrase arm-type DNA-binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimB / fimE / fimA / fimI / fimC	4754961..4803954	48993

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-109)

Antitoxin

• Antitoxin CbeA (10-110)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13060.08 Da
• Isoelectric Point: 9.4583

>T27660 WP_000691790.1 NC_017664:4770919-4771260 [Escherichia coli W]
MKIIPATTLRATTSYLSPVVVWQTLLARLLEQHYGLNLNDTPFNNEKVIQEHIDAGITLVDAVNFLVEKYELIRIDRKGF
SWQEQTPYLRAVDILRARQATGLLRRCYNTTAR

Nucleotide

• Length: 342 bp

>T27660 NC_017664:4770919-4771260 [Escherichia coli W]
ATGAAAATTATACCTGCAACGACGCTGCGGGCGACGACGTCATATCTGTCGCCTGTCGTGGTCTGGCAAACATTACTGGC
CCGCCTGCTGGAGCAGCACTACGGGCTGAATCTTAATGACACACCATTCAACAATGAAAAGGTGATTCAGGAACACATAG
ACGCCGGGATCACTCTGGTCGATGCCGTCAATTTTCTGGTGGAGAAGTACGAATTGATCCGTATCGACAGGAAAGGATTT
AGCTGGCAGGAACAGACGCCTTATCTTCGTGCGGTCGATATTTTGCGGGCGCGGCAGGCTACAGGTCTGCTGCGCCGCTG
CTATAACACCACGGCACGGTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12639.63 Da
• Isoelectric Point: 7.7723

>AT27660 WP_000939438.1 NC_017664:4770553-4770888 [Escherichia coli W]
MLQKDKVMQQEWGLPCTVTPRFTARLVQERHNLHYLADRAAITGKFSSPDLLHIDQAFPVLMKQLELKLADGELSPWQQR
TVTLYAKGLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 336 bp

>AT27660 NC_017664:4770553-4770888 [Escherichia coli W]
ATGCTGCAAAAGGACAAGGTCATGCAACAAGAATGGGGGCTTCCCTGTACCGTAACACCACGCTTCACTGCTCGACTGGT
ACAGGAAAGACATAACCTGCATTACCTGGCTGATCGGGCCGCAATCACCGGGAAATTCAGCTCCCCGGATCTGCTGCATA
TCGACCAGGCTTTTCCGGTGCTGATGAAACAACTGGAGCTGAAACTGGCTGACGGTGAACTCTCCCCCTGGCAACAACGT
ACCGTCACGCTATACGCCAAAGGACTAACCTGCGAAGCCGATACGCTGGGTTCCTGTGGTTACGTCTATATCGCTATTTA
TCCCACTCAACGCTGA

Structures

Toxin

• AlphaFold DB: E0J2C8