Detailed information of TA system TA276581

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 4556875..4558589
• Replicon: chromosome
• Accession: NZ_CP121801
• Organism: Aeromonas dhakensis strain BOND 19077

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: -
• Locus tag: QBS22_RS20910
• Protein ID: WP_201883064.1
• Coordinates: 4557936..4558589 (-)
• Length: 218 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: -
• Locus tag: QBS22_RS20905
• Protein ID: WP_201883062.1
• Coordinates: 4556875..4557939 (-)
• Length: 355 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QBS22_RS20890	4552955..4553095	-	141	WP_279484735.1	M48 family metallopeptidase	-
QBS22_RS20895	4553058..4553669	-	612	WP_279484736.1	SprT family zinc-dependent metalloprotease	-
QBS22_RS20900	4553669..4556800	-	3132	WP_201883968.1	HsdR family type I site-specific deoxyribonuclease	-
QBS22_RS20905	4556875..4557939	-	1065	WP_201883062.1	macro domain-containing protein	Antitoxin
QBS22_RS20910	4557936..4558589	-	654	WP_201883064.1	DUF4433 domain-containing protein	Toxin
QBS22_RS20915	4558692..4560158	-	1467	WP_279484738.1	restriction endonuclease subunit S	-
QBS22_RS20920	4560158..4560643	-	486	WP_279484739.1	hypothetical protein	-
QBS22_RS20925	4560576..4561169	-	594	WP_279484740.1	hypothetical protein	-
QBS22_RS20930	4561117..4562607	-	1491	WP_279484741.1	class I SAM-dependent DNA methyltransferase	-
QBS22_RS20935	4562793..4563239	-	447	WP_049636665.1	hypothetical protein	-
QBS22_RS20940	4563386..4563574	-	189	WP_021138010.1	DUF3283 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4534099..4572385	38286

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (17-216)

Antitoxin

• Macro domain (18-131)

Sequences

Toxin

Protein

• Length: 218 a.a.
• Molecular weight: 25316.92 Da
• Isoelectric Point: 7.0173

>T276581 WP_201883064.1 NZ_CP121801:c4558589-4557936 [Aeromonas dhakensis]
MAYDYSQSLNPQKALIFRIVHRDNLEWILTHGLWAGNGQQQNPHWVHIGLPELIAKRARHPVPLAPYGVLNDYVPFYFTP
FSPMLSNIHSGRGVPRRENDEILILVSSLHRMAELGLPFVFTDSHAYYQWANFYVDLGDLGRIDWPLLQRRDFKRDADDL
SKLERYQAEALIHQHCPIEALLGVVCYNEEIKNSVEQCMNRVGIARPVYVRDGWYFR

Nucleotide

• Length: 654 bp

Antitoxin

Protein

• Length: 355 a.a.
• Molecular weight: 39647.79 Da
• Isoelectric Point: 6.4968

>AT276581 WP_201883062.1 NZ_CP121801:c4557939-4556875 [Aeromonas dhakensis]
MIRYTTGNLLDAPVEALVNTVNTVGVMGKGIALMFKERFPANMRAYAEACKQCDVVTGKMFITQTGELLGPRWIVNFPTK
QHWRNPAQMAWIQDGLQDLRAWLQAQQVSSIAIPPLGAGNGGLPWPEVKQAIETALGDLDIDILLYEPSSQYQNVAKRGG
VAELTPARALIAELVRRYWFLGMECSLLEIQKLAWFLARVIGRNPLGDPLQLQFKAHRYGPYANNLTHLLNAMDGSYLQS
DKRIPDSKPFDVIWFNEAKKQELAVYLQTEAKAFLPILEQTTALIDGFESPYGMELLATVDWLIAEEGCAPTLSALREGL
HRWPAGTPWGERKARLFDDRSLQIALNRLQQGLV

Nucleotide

• Length: 1065 bp