Detailed information of TA system TA276543

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 4426081..4426660
• Replicon: chromosome
• Accession: NZ_CP121700
• Organism: Methylobacterium indicum strain JDJ13

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: QBE04_RS20450
• Protein ID: WP_279356634.1
• Coordinates: 4426081..4426353 (+)
• Length: 91 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: QBE04_RS20455
• Protein ID: WP_279356635.1
• Coordinates: 4426346..4426660 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QBE04_RS20440	4421897..4424638	-	2742	WP_279356632.1	ATP-binding cassette domain-containing protein	-
QBE04_RS20445	4425163..4425867	+	705	WP_279356633.1	carbonic anhydrase	-
QBE04_RS20450	4426081..4426353	+	273	WP_279356634.1	BrnT family toxin	Toxin
QBE04_RS20455	4426346..4426660	+	315	WP_279356635.1	BrnA antitoxin family protein	Antitoxin
QBE04_RS20460	4426776..4428191	+	1416	WP_279356636.1	DHA2 family efflux MFS transporter permease subunit	-
QBE04_RS20465	4428338..4428679	+	342	WP_279356637.1	hypothetical protein	-
QBE04_RS20470	4428705..4429328	+	624	WP_279356638.1	LpxA family transferase	-
QBE04_RS20475	4429372..4429779	-	408	WP_279360335.1	GNAT family N-acetyltransferase	-
QBE04_RS20480	4429833..4431140	-	1308	WP_279356639.1	2-oxo acid dehydrogenase subunit E2	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-83)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (26-97)

Sequences

Toxin

Protein

• Length: 91 a.a.
• Molecular weight: 10281.80 Da
• Isoelectric Point: 8.7076

>T276543 WP_279356634.1 NZ_CP121700:4426081-4426353 [Methylobacterium indicum]
MRIVWDEPKREMNLSKHGLDFADARDRFVFEDATILPSYPAPDGRPRFVALNDLDGRLVAVVFSPLGTQALTLISLRPAN
RKERRIFENA

Nucleotide

• Length: 273 bp

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11547.07 Da
• Isoelectric Point: 4.6583

>AT276543 WP_279356635.1 NZ_CP121700:4426346-4426660 [Methylobacterium indicum]
MPKLDLPPITDEDEVRIQAGIATDPDNPELTEAQFAQARPFADVFPELATTLRRARGPQKAPTKQLVSLRLDQDVVERFK
ATGPGWQTRINEALRQVAETLPTA

Nucleotide

• Length: 315 bp