Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3233864..3234518 Replicon chromosome
Accession NC_017664
Organism Escherichia coli W

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag WFL_RS16130 Protein ID WP_000244772.1
Coordinates 3233864..3234271 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag WFL_RS16135 Protein ID WP_000354046.1
Coordinates 3234252..3234518 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
WFL_RS16110 3229821..3231554 - 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -
WFL_RS16115 3231560..3232270 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
WFL_RS16120 3232295..3233191 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
WFL_RS16125 3233303..3233824 + 522 WP_001055874.1 flavodoxin FldB -
WFL_RS16130 3233864..3234271 - 408 WP_000244772.1 protein YgfX Toxin
WFL_RS16135 3234252..3234518 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
WFL_RS16140 3234761..3235741 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
WFL_RS16145 3235818..3236477 - 660 WP_000250274.1 hemolysin III family protein -
WFL_RS16150 3236641..3236952 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
WFL_RS16155 3236997..3238430 + 1434 WP_001307385.1 6-phospho-beta-glucosidase BglA -
WFL_RS16160 3238487..3239230 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T27653 WP_000244772.1 NC_017664:c3234271-3233864 [Escherichia coli W]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T27653 NC_017664:c3234271-3233864 [Escherichia coli W]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT27653 WP_000354046.1 NC_017664:c3234518-3234252 [Escherichia coli W]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT27653 NC_017664:c3234518-3234252 [Escherichia coli W]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References