27651

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 2764382..2765007 Replicon chromosome
Accession NC_017664
Organism Escherichia coli W

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag WFL_RS13860 Protein ID WP_000911329.1
Coordinates 2764382..2764780 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag WFL_RS13865 Protein ID WP_000450524.1
Coordinates 2764780..2765007 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
WFL_RS13840 2760289..2760489 + 201 WP_000383836.1 YpfN family protein -
WFL_RS13845 2760570..2761268 - 699 WP_000679823.1 esterase -
WFL_RS13850 2761342..2763357 - 2016 WP_000829294.1 tRNA cytosine(34) acetyltransferase TmcA -
WFL_RS13855 2763372..2764235 - 864 WP_001267498.1 neutral zinc metallopeptidase -
WFL_RS13860 2764382..2764780 - 399 WP_000911329.1 tRNA(fMet)-specific endonuclease VapC Toxin
WFL_RS13865 2764780..2765007 - 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
WFL_RS13870 2765162..2765875 - 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
WFL_RS13880 2766088..2767122 - 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
WFL_RS13885 2767139..2768017 - 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
WFL_RS13890 2768163..2768735 + 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
WFL_RS13895 2768735..2769205 + 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T27651 WP_000911329.1 NC_017664:c2764780-2764382 [Escherichia coli W]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T27651 NC_017664:c2764780-2764382 [Escherichia coli W]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGATTGGAGCTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT27651 WP_000450524.1 NC_017664:c2765007-2764780 [Escherichia coli W]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT27651 NC_017664:c2765007-2764780 [Escherichia coli W]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGATGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References