Detailed information of TA system TA276505

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rlegTA/AbiEii-AbiEi
• Location: 25982..27498
• Replicon: plasmid pCB3171_4
• Accession: NZ_CP121656
• Organism: Rhizobium sp. CB3171

Toxin (Protein)

• Gene name: rlegT
• Uniprot ID: -
• Locus tag: QA648_RS35585
• Protein ID: WP_283055024.1
• Coordinates: 25982..26899 (-)
• Length: 306 a.a.

Antitoxin (Protein)

• Gene name: rlegA
• Uniprot ID: -
• Locus tag: QA648_RS35590
• Protein ID: WP_283055025.1
• Coordinates: 26896..27498 (-)
• Length: 201 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QA648_RS35570 (QA648_35570)	21724..22830	-	1107	WP_283055023.1	AAA family ATPase	-
QA648_RS35575 (QA648_35575)	23034..24560	+	1527	WP_283051926.1	IS21 family transposase	-
QA648_RS35580 (QA648_35580)	24550..25362	+	813	WP_283051923.1	IS21-like element helper ATPase IstB	-
QA648_RS35585 (QA648_35585)	25982..26899	-	918	WP_283055024.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
QA648_RS35590 (QA648_35590)	26896..27498	-	603	WP_283055025.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
QA648_RS35595 (QA648_35595)	27599..31669	+	4071	WP_283055026.1	helix-turn-helix transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..186301	186301

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (38-271)

Antitoxin

• Transcriptional regulator, AbiEi antitoxin (8-50)

Sequences

Toxin

Protein

• Length: 306 a.a.
• Molecular weight: 33983.11 Da
• Isoelectric Point: 6.5051

>T276505 WP_283055024.1 NZ_CP121656:c26899-25982 [Rhizobium sp. CB3171]
MTKIIKDKSASIRARLLMHAKQSGEEYQRTLTRFAIERLLFRLSQSGVRDRYVLKGAMLFVTWPENVFRPTGDLDLLGHG
DPDPAAIVETFQQICAVEADDGLHFDLASIKVDPVREADKYQGARVMLNGELAKAIIRVQVDIGFGDHVYPAPKLASFPS
ILPDLPTASILMYPPETVVAEKFEAMIRFGLANGRIKDFYDIWLTTQIFQFQLSTVIDAVAGTLARRGTAVPTARPEGFS
QAYATIAAEKGLWSGFLERNPPMLAPPAFPELQRQLTRFFEPVIAGLNAPGGAESRWDPVEYSWR

Nucleotide

• Length: 918 bp

Antitoxin

Protein

• Length: 201 a.a.
• Molecular weight: 22279.86 Da
• Isoelectric Point: 9.6677

>AT276505 WP_283055025.1 NZ_CP121656:c27498-26896 [Rhizobium sp. CB3171]
MNYRDSALELARSRGIVRARDFKSAGIPLVYLKRLTDSGDLVRLGRGLYQDPDRVGEHVAHDLAEATRLMANGTVCLISA
LRQHELTTQLPHAVWMMIPHKARAPISPSFPVKIVRATGDALTSGIERISVEGVDVPIYGVAKTVADCFKYRRLVGEDVA
IEALRDALRFRKTTPNELMKYAAIDRCSARMEPLIRALQE

Nucleotide

• Length: 603 bp