Detailed information of TA system TA276501

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rlegTA/AbiEii-AbiEi
• Location: 31239..32769
• Replicon: plasmid pCB3171_2
• Accession: NZ_CP121654
• Organism: Rhizobium sp. CB3171

Toxin (Protein)

• Gene name: rlegT
• Uniprot ID: -
• Locus tag: QA648_RS32235
• Protein ID: WP_283054556.1
• Coordinates: 31849..32769 (+)
• Length: 307 a.a.

Antitoxin (Protein)

• Gene name: rlegA
• Uniprot ID: -
• Locus tag: QA648_RS32230
• Protein ID: WP_283054555.1
• Coordinates: 31239..31859 (+)
• Length: 207 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QA648_RS32210 (QA648_32210)	26821..28197	+	1377	WP_283054551.1	hypothetical protein	-
QA648_RS32215 (QA648_32215)	28194..28886	-	693	WP_283054552.1	hypothetical protein	-
QA648_RS32220 (QA648_32220)	28883..30487	-	1605	WP_283054553.1	adenylosuccinate synthetase	-
QA648_RS32225 (QA648_32225)	30562..31131	+	570	WP_283054554.1	hypothetical protein	-
QA648_RS32230 (QA648_32230)	31239..31859	+	621	WP_283054555.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
QA648_RS32235 (QA648_32235)	31849..32769	+	921	WP_283054556.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
QA648_RS32240 (QA648_32240)	32808..34151	+	1344	WP_283054557.1	SIR2 family protein	-
QA648_RS32245 (QA648_32245)	34159..36270	+	2112	WP_283054558.1	DUF87 domain-containing protein	-
QA648_RS32250 (QA648_32250)	36267..37499	+	1233	WP_283054559.1	exonuclease SbcCD subunit D	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..540349	540349

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (38-260)

Antitoxin

• Transcriptional regulator, AbiEi antitoxin (15-56)

Sequences

Toxin

Protein

• Length: 307 a.a.
• Molecular weight: 33747.78 Da
• Isoelectric Point: 5.5228

>T276501 WP_283054556.1 NZ_CP121654:31849-32769 [Rhizobium sp. CB3171]
MPREPRNIGASVRARLLDRARAERSDFQILLTRYALERLLYRLSASPHRDRFILKGAMLFVTWVADPFRPTRDLDLLGHG
DGDAGAIAETFRAICAQPVTDDGVRFDVAALIAAPIREEVEYGGVRVRTTATIAGARIPIQVDIGFGDAITPAAVEIDYP
TLLDAPTPRLRAYPVETVVAEKFEALVTLGVANSRLKDFYDLWVISRTFGLRQAALVEAVQRTFERRGTALPSDIPVGLT
DEFAEAWAARWRAFLGRDRMVAAPDAFAVTIADLRVFLIPLVVGLKEERVWPPSGPWSPGAAIDEA

Nucleotide

• Length: 921 bp

Antitoxin

Protein

• Length: 207 a.a.
• Molecular weight: 23447.39 Da
• Isoelectric Point: 10.1973

>AT276501 WP_283054555.1 NZ_CP121654:31239-31859 [Rhizobium sp. CB3171]
MSSKSDQRLRALKLLEARGMLRLKEFVAEGIGPETLARLVREEAVIRPARGLYQLPDAHVEAAHTLAEAAVLVPKGVVCL
TSALQYHELTLQMPSAVWMAIDRAAWRPKIDYPPIRFVRFTGSALTEGVERHRIENVDVPITDPARTIVDCFRYRTKIGL
DVAMEGLREGLRRRRCKPDQIWRYARKARVWSIMRPYVEAMVSDAA

Nucleotide

• Length: 621 bp