Detailed information of TA system TA276480

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 2449337..2449908
• Replicon: chromosome
• Accession: NZ_CP121644
• Organism: Bradyrhizobium barranii strain CC1502

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A1L3FM63
• Locus tag: QA633_RS11195
• Protein ID: WP_063985200.1
• Coordinates: 2449337..2449690 (-)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A8T5V1Z1
• Locus tag: QA633_RS11200
• Protein ID: WP_063985199.1
• Coordinates: 2449687..2449908 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QA633_RS11175 (QA633_11175)	2445629..2445874	+	246	WP_063985204.1	hypothetical protein	-
QA633_RS11180 (QA633_11180)	2446108..2447270	+	1163	Protein_2217	crosslink repair DNA glycosylase YcaQ family protein	-
QA633_RS11185 (QA633_11185)	2447395..2447823	-	429	WP_063985202.1	DUF2809 domain-containing protein	-
QA633_RS11190 (QA633_11190)	2447904..2449235	+	1332	WP_063985201.1	MFS transporter	-
QA633_RS11195 (QA633_11195)	2449337..2449690	-	354	WP_063985200.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
QA633_RS11200 (QA633_11200)	2449687..2449908	-	222	WP_063985199.1	antitoxin MazE family protein	Antitoxin
QA633_RS11205 (QA633_11205)	2450093..2451199	+	1107	WP_028148596.1	efflux RND transporter periplasmic adaptor subunit	-
QA633_RS11210 (QA633_11210)	2451376..2454513	+	3138	WP_063985198.1	efflux RND transporter permease subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-107)

Antitoxin

• Antitoxin MazE-like (11-73)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 12602.39 Da
• Isoelectric Point: 6.3275

>T276480 WP_063985200.1 NZ_CP121644:c2449690-2449337 [Bradyrhizobium barranii]
MKRGEVWTVAGGKDYAGKPRPVVIVQDDSFDATNSITVCAFTTDETEAPLFRLKVEPNDGNGLRAPSRLMVDKITTVPKA
KVGTLVGRLDDDDLLRLNRAILVFLGLAGSQRSASAD

Nucleotide

• Length: 354 bp

>T276480 NZ_CP121644:c2449690-2449337 [Bradyrhizobium barranii]
ATGAAGCGAGGTGAGGTCTGGACGGTGGCAGGCGGCAAGGACTATGCGGGCAAGCCGCGCCCCGTCGTCATCGTGCAGGA
CGACAGCTTTGACGCGACGAACTCCATCACTGTCTGCGCCTTCACGACCGACGAAACCGAGGCGCCACTTTTCCGGCTCA
AGGTCGAGCCGAACGACGGCAACGGCCTTCGAGCTCCGTCTCGACTTATGGTCGACAAGATTACTACAGTGCCAAAAGCC
AAGGTAGGAACGTTAGTGGGACGCCTAGACGATGACGACCTGCTGCGTCTCAATCGCGCTATTCTGGTGTTTCTCGGACT
GGCTGGTTCGCAGAGAAGCGCCAGTGCAGATTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8284.21 Da
• Isoelectric Point: 7.3202

>AT276480 WP_063985199.1 NZ_CP121644:c2449908-2449687 [Bradyrhizobium barranii]
MSPSKPKPSRMKVQAHRDRLRAQGLRPIQIWVPDIRSPSFQSEAHRQSLAVATSSHESEDQGFIDAVSDWTDE

Nucleotide

• Length: 222 bp

>AT276480 NZ_CP121644:c2449908-2449687 [Bradyrhizobium barranii]
ATGAGCCCTTCAAAGCCCAAGCCGTCTCGTATGAAGGTGCAGGCCCACCGCGATCGATTGCGTGCACAAGGCCTGCGGCC
GATCCAGATCTGGGTGCCGGATATCCGCTCGCCCTCCTTCCAATCGGAAGCCCATCGCCAATCGCTTGCGGTCGCAACGA
GCTCACATGAATCCGAAGACCAAGGCTTCATTGATGCCGTATCCGATTGGACCGATGAATGA

Structures

Toxin

• AlphaFold DB: A0A1L3FM63