Detailed information of TA system TA027641

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 1781916..1782442
• Replicon: chromosome
• Accession: NC_017664
• Organism: Escherichia coli W

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: WFL_RS08820
• Protein ID: WP_000323025.1
• Coordinates: 1781916..1782203 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: WFL_RS08825
• Protein ID: WP_000534858.1
• Coordinates: 1782203..1782442 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WFL_RS08780	1776940..1777155	-	216	WP_000839590.1	phage lysis protein EssD	-
WFL_RS08785	1777909..1778124	-	216	WP_000066484.1	cold shock-like protein CspB	-
WFL_RS08790	1778425..1778637	+	213	WP_000087756.1	cold shock-like protein CspF	-
WFL_RS27395	1778692..1778781	+	90	WP_120795389.1	hypothetical protein	-
WFL_RS08795	1779059..1779811	-	753	WP_001047135.1	antitermination protein	-
WFL_RS08800	1779825..1780874	-	1050	WP_001265199.1	DUF968 domain-containing protein	-
WFL_RS08805	1780876..1781154	-	279	WP_012304870.1	hypothetical protein	-
WFL_RS08810	1781221..1781472	-	252	WP_000980994.1	hypothetical protein	-
WFL_RS08815	1781689..1781844	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
WFL_RS08820	1781916..1782203	-	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
WFL_RS08825	1782203..1782442	-	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
WFL_RS25720	1782467..1782772	+	306	WP_001326990.1	hypothetical protein	-
WFL_RS08830	1782975..1783307	+	333	WP_001301033.1	protein FlxA	-
WFL_RS08835	1783744..1785057	-	1314	WP_000589005.1	ISNCY family transposase	-
WFL_RS27780	1785826..1786113	-	288	Protein_1686	hypothetical protein	-
WFL_RS08850	1786724..1787080	-	357	WP_001310834.1	class I SAM-dependent methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1746516..1795565	49049

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T27641 WP_000323025.1 NC_017664:c1782203-1781916 [Escherichia coli W]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T27641 NC_017664:c1782203-1781916 [Escherichia coli W]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT27641 WP_000534858.1 NC_017664:c1782442-1782203 [Escherichia coli W]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT27641 NC_017664:c1782442-1782203 [Escherichia coli W]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8