Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 1611910..1612548 Replicon chromosome
Accession NC_017664
Organism Escherichia coli W

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag WFL_RS08005 Protein ID WP_000813794.1
Coordinates 1611910..1612086 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag WFL_RS08010 Protein ID WP_001270285.1
Coordinates 1612132..1612548 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
WFL_RS07985 1607529..1608743 - 1215 WP_001326689.1 BenE family transporter YdcO -
WFL_RS07990 1608796..1609332 + 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
WFL_RS07995 1609405..1611366 + 1962 WP_001301045.1 23S rRNA 5-hydroxycytidine C2501 synthase -
WFL_RS08000 1611458..1611688 - 231 WP_000494244.1 YncJ family protein -
WFL_RS08005 1611910..1612086 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
WFL_RS08010 1612132..1612548 + 417 WP_001270285.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
WFL_RS08015 1612627..1614033 + 1407 WP_000760589.1 PLP-dependent aminotransferase family protein -
WFL_RS08020 1614278..1615423 + 1146 WP_000047456.1 ABC transporter substrate-binding protein -
WFL_RS08025 1615441..1616454 + 1014 WP_000220396.1 ABC transporter ATP-binding protein -
WFL_RS08030 1616455..1617396 + 942 WP_001251315.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 1606341..1607489 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(88-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T27639 WP_000813794.1 NC_017664:1611910-1612086 [Escherichia coli W]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T27639 NC_017664:1611910-1612086 [Escherichia coli W]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15247.61 Da        Isoelectric Point: 4.6115

>AT27639 WP_001270285.1 NC_017664:1612132-1612548 [Escherichia coli W]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFDLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT27639 NC_017664:1612132-1612548 [Escherichia coli W]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTGACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References