Detailed information of TA system TA027639

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1611910..1612548
• Replicon: chromosome
• Accession: NC_017664
• Organism: Escherichia coli W

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: WFL_RS08005
• Protein ID: WP_000813794.1
• Coordinates: 1611910..1612086 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: WFL_RS08010
• Protein ID: WP_001270285.1
• Coordinates: 1612132..1612548 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WFL_RS07985	1607529..1608743	-	1215	WP_001326689.1	BenE family transporter YdcO	-
WFL_RS07990	1608796..1609332	+	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
WFL_RS07995	1609405..1611366	+	1962	WP_001301045.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
WFL_RS08000	1611458..1611688	-	231	WP_000494244.1	YncJ family protein	-
WFL_RS08005	1611910..1612086	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
WFL_RS08010	1612132..1612548	+	417	WP_001270285.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
WFL_RS08015	1612627..1614033	+	1407	WP_000760589.1	PLP-dependent aminotransferase family protein	-
WFL_RS08020	1614278..1615423	+	1146	WP_000047456.1	ABC transporter substrate-binding protein	-
WFL_RS08025	1615441..1616454	+	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
WFL_RS08030	1616455..1617396	+	942	WP_001251315.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1606341..1607489	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (88-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T27639 WP_000813794.1 NC_017664:1611910-1612086 [Escherichia coli W]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T27639 NC_017664:1611910-1612086 [Escherichia coli W]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15247.61 Da
• Isoelectric Point: 4.6115

>AT27639 WP_001270285.1 NC_017664:1612132-1612548 [Escherichia coli W]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFDLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT27639 NC_017664:1612132-1612548 [Escherichia coli W]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTGACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB