Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 475660..476297 Replicon chromosome
Accession NZ_CP121465
Organism Bacillus velezensis strain YA215

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag P9972_RS02465 Protein ID WP_003156187.1
Coordinates 475947..476297 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag P9972_RS02460 Protein ID WP_003156188.1
Coordinates 475660..475941 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P9972_RS02440 (P9972_02440) 472025..472624 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
P9972_RS02445 (P9972_02445) 472717..473082 + 366 WP_014304402.1 holo-ACP synthase -
P9972_RS02450 (P9972_02450) 473247..474254 + 1008 WP_003156191.1 outer membrane lipoprotein carrier protein LolA -
P9972_RS02455 (P9972_02455) 474371..475540 + 1170 WP_003156189.1 alanine racemase -
P9972_RS02460 (P9972_02460) 475660..475941 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
P9972_RS02465 (P9972_02465) 475947..476297 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
P9972_RS02470 (P9972_02470) 476415..477236 + 822 WP_003156182.1 STAS domain-containing protein -
P9972_RS02475 (P9972_02475) 477241..477606 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
P9972_RS02480 (P9972_02480) 477609..478010 + 402 WP_003156178.1 anti-sigma regulatory factor -
P9972_RS02485 (P9972_02485) 478022..479029 + 1008 WP_003156177.1 PP2C family protein-serine/threonine phosphatase -
P9972_RS02490 (P9972_02490) 479093..479422 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
P9972_RS02495 (P9972_02495) 479419..479901 + 483 WP_003156173.1 anti-sigma B factor RsbW -
P9972_RS02500 (P9972_02500) 479867..480655 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
P9972_RS02505 (P9972_02505) 480655..481257 + 603 WP_003156170.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T276348 WP_003156187.1 NZ_CP121465:475947-476297 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT276348 WP_003156188.1 NZ_CP121465:475660-475941 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References