Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4572962..4573538 Replicon chromosome
Accession NZ_CP121356
Organism Escherichia coli strain rl0044

Toxin (Protein)


Gene name shpA Uniprot ID A0A2X2XYK1
Locus tag P9060_RS22985 Protein ID WP_001360091.1
Coordinates 4573251..4573538 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag P9060_RS22980 Protein ID WP_000063156.1
Coordinates 4572962..4573264 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P9060_RS22965 (4569558) 4569558..4571708 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
P9060_RS22970 (4571758) 4571758..4571961 + 204 WP_000467859.1 YbdD/YjiX family protein -
P9060_RS22975 (4571972) 4571972..4572928 + 957 WP_001297640.1 GTPase -
P9060_RS22980 (4572962) 4572962..4573264 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
P9060_RS22985 (4573251) 4573251..4573538 - 288 WP_001360091.1 BrnT family toxin Toxin
P9060_RS22990 (4573737) 4573737..4574434 - 698 Protein_4503 IS1-like element IS1F family transposase -
P9060_RS22995 (4574491) 4574491..4574586 + 96 Protein_4504 dienelactone hydrolase family protein -
P9060_RS23000 (4574732) 4574732..4575964 + 1233 WP_015675357.1 multidrug efflux MFS transporter MdtM -
P9060_RS23005 (4576005) 4576005..4577285 + 1281 WP_001037377.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11325.80 Da        Isoelectric Point: 6.8821

>T276312 WP_001360091.1 NZ_CP121356:c4573538-4573251 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKTDRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT276312 WP_000063156.1 NZ_CP121356:c4573264-4572962 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2X2XYK1

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References