Detailed information of TA system TA276280

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 4730361..4730925
• Replicon: chromosome
• Accession: NZ_CP121354
• Organism: Mesorhizobium sp. WSM4904

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: QAZ47_RS22760
• Protein ID: WP_278202991.1
• Coordinates: 4730668..4730925 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: QAZ47_RS22755
• Protein ID: WP_278202990.1
• Coordinates: 4730361..4730681 (-)
• Length: 107 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QAZ47_RS22720 (QAZ47_22720)	4725396..4726271	+	876	WP_278207913.1	zinc ABC transporter permease AztB	-
QAZ47_RS22725 (QAZ47_22725)	4726301..4727242	+	942	WP_278202986.1	zinc ABC transporter substrate-binding protein AztC	-
QAZ47_RS22730 (QAZ47_22730)	4727389..4727727	+	339	WP_278207914.1	TIGR01244 family sulfur transferase	-
QAZ47_RS22735 (QAZ47_22735)	4727960..4728427	-	468	WP_059187534.1	Lrp/AsnC family transcriptional regulator	-
QAZ47_RS22740 (QAZ47_22740)	4728566..4729681	+	1116	WP_278202987.1	4-hydroxyphenylpyruvate dioxygenase	-
QAZ47_RS22745 (QAZ47_22745)	4729751..4729975	+	225	WP_278202988.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
QAZ47_RS22750 (QAZ47_22750)	4729972..4730349	+	378	WP_278202989.1	type II toxin-antitoxin system VapC family toxin	-
QAZ47_RS22755 (QAZ47_22755)	4730361..4730681	-	321	WP_278202990.1	BrnA antitoxin family protein	Antitoxin
QAZ47_RS22760 (QAZ47_22760)	4730668..4730925	-	258	WP_278202991.1	BrnT family toxin	Toxin
QAZ47_RS22765 (QAZ47_22765)	4731002..4731538	-	537	WP_278230802.1	DinB family protein	-
QAZ47_RS22770 (QAZ47_22770)	4731543..4732568	-	1026	WP_278230803.1	fumarylacetoacetate hydrolase family protein	-
QAZ47_RS22775 (QAZ47_22775)	4732565..4733467	-	903	WP_278202994.1	MBL fold metallo-hydrolase	-
QAZ47_RS22780 (QAZ47_22780)	4733464..4734777	-	1314	WP_278230804.1	homogentisate 1,2-dioxygenase	-
QAZ47_RS22785 (QAZ47_22785)	4734911..4735357	+	447	WP_278233849.1	MarR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-77)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (31-104)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9346.93 Da
• Isoelectric Point: 10.1808

>T276280 WP_278202991.1 NZ_CP121354:c4730925-4730668 [Mesorhizobium sp. WSM4904]
MKIVWDEPKRLANIDKHGLDFAALDEEFFLASTIRAAKAGRFMAIGWIVGGVVAVVFARLGSEGISIISMRPANQAERRL
FDGKV

Nucleotide

• Length: 258 bp

>T276280 NZ_CP121354:c4730925-4730668 [Mesorhizobium sp. WSM4904]
ATGAAGATCGTTTGGGATGAACCGAAACGGTTGGCTAACATCGACAAACACGGCTTGGATTTCGCGGCGTTGGACGAGGA
GTTTTTCCTTGCGTCGACGATCCGGGCGGCCAAGGCCGGTCGGTTCATGGCGATTGGTTGGATAGTCGGCGGCGTTGTTG
CAGTGGTCTTTGCCCGACTTGGCTCCGAGGGCATTTCGATCATTTCGATGCGGCCGGCCAACCAGGCGGAAAGGAGATTG
TTCGATGGCAAAGTCTAA

Antitoxin

Protein

• Length: 107 a.a.
• Molecular weight: 12114.65 Da
• Isoelectric Point: 10.4726

>AT276280 WP_278202990.1 NZ_CP121354:c4730681-4730361 [Mesorhizobium sp. WSM4904]
MAKSKLRIKKPDKEFESGKGFTREDWDAVSDNPEWTEEDFRNARPFAEVFPDLADSIRRSRGRPALDNPKKQVTLRLDSD
VIARFRAGGPGWQSRINDILRKAAGL

Nucleotide

• Length: 321 bp

>AT276280 NZ_CP121354:c4730681-4730361 [Mesorhizobium sp. WSM4904]
ATGGCAAAGTCTAAGCTACGCATCAAGAAGCCCGACAAGGAATTCGAGTCAGGCAAGGGCTTCACCAGGGAAGACTGGGA
TGCGGTTTCAGACAATCCCGAATGGACCGAGGAGGACTTTCGGAATGCTCGTCCCTTCGCCGAGGTGTTTCCGGATCTTG
CCGACAGCATTCGCCGTTCGCGCGGCCGGCCGGCGCTCGACAATCCGAAGAAACAAGTGACGCTGCGTCTCGATAGCGAC
GTCATCGCCCGCTTCCGCGCCGGCGGACCCGGCTGGCAGAGCCGCATCAACGACATTTTGCGGAAAGCCGCCGGCCTTTG
A