Detailed information of TA system TA027628

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 4558796..4559633
• Replicon: chromosome
• Accession: NC_017660
• Organism: Escherichia coli KO11FL

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: KO11_RS22460
• Protein ID: WP_000227784.1
• Coordinates: 4559091..4559633 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: KO11_RS22455
• Protein ID: WP_001297137.1
• Coordinates: 4558796..4559107 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KO11_RS22430	4553816..4554763	+	948	WP_001239440.1	cytochrome o ubiquinol oxidase subunit II	-
KO11_RS22435	4554785..4556776	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
KO11_RS22440	4556766..4557380	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
KO11_RS22445	4557380..4557709	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
KO11_RS22450	4557721..4558611	+	891	WP_000971336.1	heme o synthase	-
KO11_RS22455	4558796..4559107	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
KO11_RS22460	4559091..4559633	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
KO11_RS22465	4559689..4560613	-	925	Protein_4303	sel1 repeat family protein	-
KO11_RS22470	4561021..4562385	+	1365	WP_001000978.1	MFS transporter	-
KO11_RS22475	4562513..4563004	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
KO11_RS22480	4563172..4564083	+	912	WP_000705849.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T27628 WP_000227784.1 NC_017660:4559091-4559633 [Escherichia coli KO11FL]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T27628 NC_017660:4559091-4559633 [Escherichia coli KO11FL]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT27628 WP_001297137.1 NC_017660:4558796-4559107 [Escherichia coli KO11FL]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT27628 NC_017660:4558796-4559107 [Escherichia coli KO11FL]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3