Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4524719..4525337 Replicon chromosome
Accession NC_017660
Organism Escherichia coli KO11FL

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag KO11_RS22290 Protein ID WP_001291435.1
Coordinates 4525119..4525337 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag KO11_RS22285 Protein ID WP_000344800.1
Coordinates 4524719..4525093 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KO11_RS22275 4519808..4521001 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KO11_RS22280 4521024..4524173 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
KO11_RS22285 4524719..4525093 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
KO11_RS22290 4525119..4525337 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
KO11_RS22295 4525509..4526060 + 552 WP_000102564.1 maltose O-acetyltransferase -
KO11_RS22300 4526176..4526646 + 471 WP_000136192.1 YlaC family protein -
KO11_RS22305 4526810..4528360 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
KO11_RS22310 4528402..4528755 - 354 WP_000878140.1 DUF1428 domain-containing protein -
KO11_RS22315 4529134..4529445 + 312 WP_000409911.1 MGMT family protein -
KO11_RS22320 4529476..4530048 - 573 WP_000779826.1 lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T27627 WP_001291435.1 NC_017660:4525119-4525337 [Escherichia coli KO11FL]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T27627 NC_017660:4525119-4525337 [Escherichia coli KO11FL]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT27627 WP_000344800.1 NC_017660:4524719-4525093 [Escherichia coli KO11FL]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT27627 NC_017660:4524719-4525093 [Escherichia coli KO11FL]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References