Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 3265404..3266042 Replicon chromosome
Accession NC_017660
Organism Escherichia coli KO11FL

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag KO11_RS16185 Protein ID WP_000813794.1
Coordinates 3265404..3265580 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag KO11_RS16190 Protein ID WP_001270285.1
Coordinates 3265626..3266042 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KO11_RS16165 3261023..3262237 - 1215 WP_001326689.1 BenE family transporter YdcO -
KO11_RS16170 3262290..3262826 + 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
KO11_RS16175 3262899..3264860 + 1962 WP_001301045.1 23S rRNA 5-hydroxycytidine C2501 synthase -
KO11_RS16180 3264952..3265182 - 231 WP_000494244.1 YncJ family protein -
KO11_RS16185 3265404..3265580 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
KO11_RS16190 3265626..3266042 + 417 WP_001270285.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
KO11_RS16195 3266121..3267527 + 1407 WP_000760593.1 PLP-dependent aminotransferase family protein -
KO11_RS16200 3267772..3268917 + 1146 WP_000047456.1 ABC transporter substrate-binding protein -
KO11_RS16205 3268935..3269948 + 1014 WP_000220396.1 ABC transporter ATP-binding protein -
KO11_RS16210 3269949..3270890 + 942 WP_001251315.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3259718..3260983 1265


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(88-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T27625 WP_000813794.1 NC_017660:3265404-3265580 [Escherichia coli KO11FL]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T27625 NC_017660:3265404-3265580 [Escherichia coli KO11FL]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15247.61 Da        Isoelectric Point: 4.6115

>AT27625 WP_001270285.1 NC_017660:3265626-3266042 [Escherichia coli KO11FL]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFDLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT27625 NC_017660:3265626-3266042 [Escherichia coli KO11FL]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTGACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References