Detailed information of TA system TA027624

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 3175606..3176132
• Replicon: chromosome
• Accession: NC_017660
• Organism: Escherichia coli KO11FL

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: KO11_RS15710
• Protein ID: WP_000323025.1
• Coordinates: 3175845..3176132 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: KO11_RS15705
• Protein ID: WP_000534858.1
• Coordinates: 3175606..3175845 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KO11_RS15680	3170968..3171324	+	357	WP_001310834.1	class I SAM-dependent methyltransferase	-
KO11_RS28205	3171935..3172222	+	288	Protein_2970	hypothetical protein	-
KO11_RS15695	3172991..3174304	+	1314	WP_000589005.1	ISNCY family transposase	-
KO11_RS15700	3174741..3175073	-	333	WP_001301033.1	protein FlxA	-
KO11_RS25995	3175276..3175581	-	306	WP_001326990.1	hypothetical protein	-
KO11_RS15705	3175606..3175845	+	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
KO11_RS15710	3175845..3176132	+	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
KO11_RS15715	3176204..3176359	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
KO11_RS15720	3176576..3176827	+	252	WP_000980994.1	hypothetical protein	-
KO11_RS15725	3176894..3177172	+	279	WP_012304870.1	hypothetical protein	-
KO11_RS15730	3177174..3178223	+	1050	WP_001265199.1	DUF968 domain-containing protein	-
KO11_RS15735	3178237..3178989	+	753	WP_001047135.1	antitermination protein	-
KO11_RS27805	3179267..3179356	-	90	WP_120795389.1	hypothetical protein	-
KO11_RS15740	3179411..3179623	-	213	WP_000087756.1	cold shock-like protein CspF	-
KO11_RS15745	3179924..3180139	+	216	WP_000066484.1	cold shock-like protein CspB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3155983..3211440	55457
	-	flank	IS/Tn	-	-	3180555..3181763	1208

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T27624 WP_000323025.1 NC_017660:3175845-3176132 [Escherichia coli KO11FL]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T27624 NC_017660:3175845-3176132 [Escherichia coli KO11FL]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT27624 WP_000534858.1 NC_017660:3175606-3175845 [Escherichia coli KO11FL]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT27624 NC_017660:3175606-3175845 [Escherichia coli KO11FL]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8