Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1730960..1731543 Replicon chromosome
Accession NZ_CP121294
Organism Escherichia coli O155:H21 strain NWU_1

Toxin (Protein)


Gene name mazF Uniprot ID U9XFN8
Locus tag P9N54_RS08565 Protein ID WP_000254745.1
Coordinates 1730960..1731295 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag P9N54_RS08570 Protein ID WP_000581937.1
Coordinates 1731295..1731543 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P9N54_RS08545 (1726429) 1726429..1726791 + 363 WP_000034929.1 hypothetical protein -
P9N54_RS08550 (1726847) 1726847..1728145 - 1299 WP_000036723.1 phosphopyruvate hydratase -
P9N54_RS08555 (1728233) 1728233..1729870 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
P9N54_RS08560 (1730098) 1730098..1730889 - 792 WP_136721162.1 nucleoside triphosphate pyrophosphohydrolase -
P9N54_RS08565 (1730960) 1730960..1731295 - 336 WP_000254745.1 endoribonuclease MazF Toxin
P9N54_RS08570 (1731295) 1731295..1731543 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
P9N54_RS08575 (1731621) 1731621..1733855 - 2235 WP_000226815.1 GTP pyrophosphokinase -
P9N54_RS08580 (1733903) 1733903..1735204 - 1302 WP_000046790.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T276195 WP_000254745.1 NZ_CP121294:c1731295-1730960 [Escherichia coli O155:H21]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT276195 WP_000581937.1 NZ_CP121294:c1731543-1731295 [Escherichia coli O155:H21]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYM3

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References