Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 1482854..1483691 Replicon chromosome
Accession NZ_CP121294
Organism Escherichia coli O155:H21 strain NWU_1

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag P9N54_RS07335 Protein ID WP_000227784.1
Coordinates 1482854..1483396 (-) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag P9N54_RS07340 Protein ID WP_001297137.1
Coordinates 1483380..1483691 (-) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P9N54_RS07315 (1478393) 1478393..1479304 - 912 WP_000705847.1 2-dehydropantoate 2-reductase -
P9N54_RS07320 (1479472) 1479472..1479963 + 492 WP_001138904.1 nucleotide binding protein YajQ -
P9N54_RS07325 (1480091) 1480091..1481455 - 1365 WP_001000978.1 MFS transporter -
P9N54_RS07330 (1481863) 1481863..1482798 + 936 WP_001297127.1 tetratricopeptide repeat protein -
P9N54_RS07335 (1482854) 1482854..1483396 - 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
P9N54_RS07340 (1483380) 1483380..1483691 - 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
P9N54_RS07345 (1483876) 1483876..1484766 - 891 WP_000971336.1 heme o synthase -
P9N54_RS07350 (1484778) 1484778..1485107 - 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
P9N54_RS07355 (1485107) 1485107..1485721 - 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
P9N54_RS07360 (1485711) 1485711..1487702 - 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
P9N54_RS07365 (1487724) 1487724..1488671 - 948 WP_001239441.1 cytochrome o ubiquinol oxidase subunit II -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T276193 WP_000227784.1 NZ_CP121294:c1483396-1482854 [Escherichia coli O155:H21]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT276193 WP_001297137.1 NZ_CP121294:c1483691-1483380 [Escherichia coli O155:H21]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References