Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 508833..509469 Replicon chromosome
Accession NZ_CP121266
Organism Bacillus subtilis strain 107105

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag P8R49_RS02615 Protein ID WP_003156187.1
Coordinates 509119..509469 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag P8R49_RS02610 Protein ID WP_003225183.1
Coordinates 508833..509114 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P8R49_RS02590 (P8R49_02590) 505192..505791 - 600 WP_014478896.1 rhomboid family intramembrane serine protease -
P8R49_RS02595 (P8R49_02595) 505886..506251 + 366 WP_014478897.1 holo-ACP synthase -
P8R49_RS02600 (P8R49_02600) 506417..507433 + 1017 WP_003234282.1 outer membrane lipoprotein carrier protein LolA -
P8R49_RS02605 (P8R49_02605) 507548..508717 + 1170 WP_014478898.1 alanine racemase -
P8R49_RS02610 (P8R49_02610) 508833..509114 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
P8R49_RS02615 (P8R49_02615) 509119..509469 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
P8R49_RS02620 (P8R49_02620) 509585..510409 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
P8R49_RS02625 (P8R49_02625) 510414..510779 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
P8R49_RS02630 (P8R49_02630) 510783..511184 + 402 WP_014478899.1 serine/threonine-protein kinase RsbT -
P8R49_RS02635 (P8R49_02635) 511196..512203 + 1008 WP_014478900.1 phosphoserine phosphatase RsbU -
P8R49_RS02640 (P8R49_02640) 512272..512601 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
P8R49_RS02645 (P8R49_02645) 512598..513080 + 483 WP_003234299.1 anti-sigma B factor RsbW -
P8R49_RS02650 (P8R49_02650) 513046..513834 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
P8R49_RS02655 (P8R49_02655) 513834..514433 + 600 WP_003246608.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T276157 WP_003156187.1 NZ_CP121266:509119-509469 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT276157 WP_003225183.1 NZ_CP121266:508833-509114 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References