Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 2227601..2228226 Replicon chromosome
Accession NC_017660
Organism Escherichia coli KO11FL

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag KO11_RS10980 Protein ID WP_000911329.1
Coordinates 2227828..2228226 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag KO11_RS10975 Protein ID WP_000450524.1
Coordinates 2227601..2227828 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KO11_RS10945 2223403..2223873 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
KO11_RS10950 2223873..2224445 - 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
KO11_RS10955 2224591..2225469 + 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
KO11_RS10960 2225486..2226520 + 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
KO11_RS10970 2226733..2227446 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
KO11_RS10975 2227601..2227828 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
KO11_RS10980 2227828..2228226 + 399 WP_000911329.1 tRNA(fMet)-specific endonuclease VapC Toxin
KO11_RS10985 2228373..2229236 + 864 WP_001267505.1 neutral zinc metallopeptidase -
KO11_RS10990 2229251..2231266 + 2016 WP_000829294.1 tRNA cytosine(34) acetyltransferase TmcA -
KO11_RS10995 2231340..2232038 + 699 WP_000679823.1 esterase -
KO11_RS11000 2232119..2232319 - 201 WP_000383836.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T27614 WP_000911329.1 NC_017660:2227828-2228226 [Escherichia coli KO11FL]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T27614 NC_017660:2227828-2228226 [Escherichia coli KO11FL]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGATTGGAGCTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT27614 WP_000450524.1 NC_017660:2227601-2227828 [Escherichia coli KO11FL]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT27614 NC_017660:2227601-2227828 [Escherichia coli KO11FL]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGATGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References