Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1754074..1754728 Replicon chromosome
Accession NC_017660
Organism Escherichia coli KO11FL

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag KO11_RS08685 Protein ID WP_000244772.1
Coordinates 1754321..1754728 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag KO11_RS08680 Protein ID WP_000354046.1
Coordinates 1754074..1754340 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KO11_RS08655 1749362..1750105 + 744 WP_000951964.1 SDR family oxidoreductase -
KO11_RS08660 1750162..1751595 - 1434 WP_001307385.1 6-phospho-beta-glucosidase BglA -
KO11_RS08665 1751640..1751951 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
KO11_RS08670 1752115..1752774 + 660 WP_000250274.1 hemolysin III family protein -
KO11_RS08675 1752851..1753831 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
KO11_RS08680 1754074..1754340 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
KO11_RS08685 1754321..1754728 + 408 WP_000244772.1 protein YgfX Toxin
KO11_RS08690 1754768..1755289 - 522 WP_001055874.1 flavodoxin FldB -
KO11_RS08695 1755401..1756297 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
KO11_RS08700 1756322..1757032 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
KO11_RS08705 1757038..1758771 + 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T27612 WP_000244772.1 NC_017660:1754321-1754728 [Escherichia coli KO11FL]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T27612 NC_017660:1754321-1754728 [Escherichia coli KO11FL]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT27612 WP_000354046.1 NC_017660:1754074-1754340 [Escherichia coli KO11FL]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT27612 NC_017660:1754074-1754340 [Escherichia coli KO11FL]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References