Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 1188143..1188943 Replicon chromosome
Accession NC_017660
Organism Escherichia coli KO11FL

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag KO11_RS05785 Protein ID WP_000342449.1
Coordinates 1188416..1188943 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag KO11_RS05780 Protein ID WP_001277108.1
Coordinates 1188143..1188409 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KO11_RS05760 1183801..1184469 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
KO11_RS05765 1184462..1185520 + 1059 WP_001042003.1 permease-like cell division protein FtsX -
KO11_RS05770 1185765..1186619 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
KO11_RS05775 1186890..1187993 + 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
KO11_RS05780 1188143..1188409 + 267 WP_001277108.1 DUF1778 domain-containing protein Antitoxin
KO11_RS05785 1188416..1188943 + 528 WP_000342449.1 GNAT family N-acetyltransferase Toxin
KO11_RS05790 1188940..1189323 - 384 WP_000778781.1 aspartate 1-decarboxylase autocleavage activator PanM -
KO11_RS05795 1189747..1190856 + 1110 WP_000827696.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
KO11_RS05800 1190904..1191830 + 927 WP_000003004.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
KO11_RS05805 1191827..1193104 + 1278 WP_000803798.1 branched chain amino acid ABC transporter permease LivM -
KO11_RS05810 1193101..1193868 + 768 WP_000082110.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T27610 WP_000342449.1 NC_017660:1188416-1188943 [Escherichia coli KO11FL]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T27610 NC_017660:1188416-1188943 [Escherichia coli KO11FL]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTTACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATAAAAAAGCCCATACGTTTTATCAATCGCTGGGCTTTATCCCGTTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATCGAACTGCTTTTCACACAGAGCGATTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT27610 WP_001277108.1 NC_017660:1188143-1188409 [Escherichia coli KO11FL]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT27610 NC_017660:1188143-1188409 [Escherichia coli KO11FL]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCTGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References