Detailed information of TA system TA027609

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 701548..702267
• Replicon: chromosome
• Accession: NC_017660
• Organism: Escherichia coli KO11FL

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: E0J373
• Locus tag: KO11_RS03465
• Protein ID: WP_001095907.1
• Coordinates: 701947..702267 (+)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: E0J374
• Locus tag: KO11_RS03460
• Protein ID: WP_001192122.1
• Coordinates: 701548..701913 (+)
• Length: 122 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KO11_RS03420	696642..696932	+	291	Protein_642	hypothetical protein	-
KO11_RS03435	698357..699160	+	804	WP_001245321.1	hypothetical protein	-
KO11_RS03440	699377..699853	+	477	WP_001254693.1	hypothetical protein	-
KO11_RS03445	699925..700746	+	822	WP_000197395.1	DUF945 domain-containing protein	-
KO11_RS03450	700816..701286	+	471	WP_000104319.1	DNA repair protein RadC	-
KO11_RS03455	701295..701516	+	222	WP_000691985.1	DUF987 family protein	-
KO11_RS03460	701548..701913	+	366	WP_001192122.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
KO11_RS03465	701947..702267	+	321	WP_001095907.1	TA system toxin CbtA family protein	Toxin
KO11_RS03475	703034..704218	+	1185	WP_001172876.1	sugar efflux transporter	-
KO11_RS03480	704329..705252	+	924	WP_000535960.1	carboxylate/amino acid/amine transporter	-
KO11_RS03485	705256..706074	-	819	WP_000779426.1	lipoprotein NlpA	-
KO11_RS03495	706296..706589	+	294	WP_000805509.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (5-104)

Antitoxin

• Antitoxin CbeA (18-116)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12184.76 Da
• Isoelectric Point: 5.5740

>T27609 WP_001095907.1 NC_017660:701947-702267 [Escherichia coli KO11FL]
MNTPTVPATVTVSSRLSPVQVWQKLLTYILEHHYGLTINDTPFSNDTTIQEHIEAGVNLTDAVNFLVERFELVRIDQKGF
SWQDQEPWITSLDVRRAQFNLGLKRS

Nucleotide

• Length: 321 bp

>T27609 NC_017660:701947-702267 [Escherichia coli KO11FL]
ATGAATACTCCAACTGTACCGGCAACGGTGACGGTTTCGTCACGCCTGTCTCCTGTCCAAGTTTGGCAAAAACTATTAAC
GTATATTTTAGAGCATCATTACGGCCTCACGATCAACGACACACCGTTCAGTAACGATACGACGATTCAGGAACATATAG
AGGCTGGAGTGAATCTTACTGATGCTGTTAATTTTCTTGTGGAACGATTCGAGCTGGTGCGCATTGACCAGAAGGGTTTC
TCCTGGCAGGATCAAGAACCCTGGATAACATCTTTGGATGTTCGTCGTGCACAGTTCAACCTTGGCTTGAAACGCTCATA
A

Antitoxin

Protein

• Length: 122 a.a.
• Molecular weight: 13470.22 Da
• Isoelectric Point: 6.8421

>AT27609 WP_001192122.1 NC_017660:701548-701913 [Escherichia coli KO11FL]
MQSTTLSGTSAENVSCLQWGLKRNITPCFGARLVQEGNRLYFLADRAGFNGSFSDDEALRLDQAFPLMMKQLERMLTAGE
LDPRSQHCVTRHHNGLTCEADTLGSHGYVYIAIYPQSALTR

Nucleotide

• Length: 366 bp

>AT27609 NC_017660:701548-701913 [Escherichia coli KO11FL]
ATGCAGTCGACCACCTTATCCGGCACATCGGCGGAGAACGTATCCTGCCTTCAGTGGGGTTTAAAACGTAATATCACGCC
ATGCTTCGGCGCTCGCCTGGTACAGGAAGGCAACCGCCTATACTTTCTGGCTGACCGGGCAGGATTTAATGGCTCATTCA
GTGACGATGAAGCTTTGCGTCTGGACCAGGCATTTCCACTGATGATGAAACAGCTTGAGCGGATGCTGACGGCTGGCGAG
CTTGATCCTCGCAGCCAGCACTGCGTCACACGGCATCACAACGGACTTACTTGTGAAGCTGACACTCTCGGCTCTCATGG
CTACGTTTACATTGCCATATATCCCCAGTCAGCTCTAACCAGATAA

Structures

Toxin

• AlphaFold DB: E0J373

Antitoxin

• AlphaFold DB: E0J374