Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 325437..326068 Replicon chromosome
Accession NZ_CP121199
Organism Escherichia coli strain E50

Toxin (Protein)


Gene name hicA Uniprot ID L4J012
Locus tag P6H83_RS01450 Protein ID WP_001260301.1
Coordinates 325437..325712 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag P6H83_RS01455 Protein ID WP_000593555.1
Coordinates 325709..326068 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P6H83_RS01435 (320441) 320441..321508 + 1068 WP_000361497.1 HlyD family efflux transporter periplasmic adaptor subunit -
P6H83_RS01440 (321505) 321505..324240 + 2736 WP_061092822.1 ribosome-associated ATPase/putative transporter RbbA -
P6H83_RS01445 (324240) 324240..325364 + 1125 WP_001314210.1 ABC transporter permease -
P6H83_RS01450 (325437) 325437..325712 + 276 WP_001260301.1 type II toxin-antitoxin system HicA family toxin Toxin
P6H83_RS01455 (325709) 325709..326068 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
P6H83_RS01460 (326188) 326188..326589 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
P6H83_RS01465 (326594) 326594..327400 - 807 WP_061092823.1 nickel import ATP-binding protein NikE -
P6H83_RS01470 (327397) 327397..328161 - 765 WP_001136211.1 nickel import ATP-binding protein NikD -
P6H83_RS01475 (328161) 328161..328994 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
P6H83_RS01480 (328991) 328991..329935 - 945 WP_000947080.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 318453..326589 8136


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10185.87 Da        Isoelectric Point: 10.6128

>T276035 WP_001260301.1 NZ_CP121199:325437-325712 [Escherichia coli]
MRTQVQALRKKQKNTLDQIFKSPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT276035 WP_000593555.1 NZ_CP121199:325709-326068 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB L4J012

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References