Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 52092..52617 Replicon plasmid pE89-1
Accession NZ_CP121158
Organism Escherichia coli strain E89

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag P6H86_RS23850 Protein ID WP_001159868.1
Coordinates 52092..52397 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag P6H86_RS23855 Protein ID WP_000813634.1
Coordinates 52399..52617 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P6H86_RS23825 (48122) 48122..48877 - 756 WP_000852146.1 replication initiation protein RepE -
P6H86_RS23830 (49597) 49597..50265 - 669 Protein_63 site-specific integrase -
P6H86_RS23835 (50437) 50437..51636 - 1200 WP_112901278.1 IS91 family transposase -
P6H86_RS23840 (51646) 51646..51834 - 189 WP_000957857.1 hypothetical protein -
P6H86_RS23845 (51951) 51951..52091 - 141 Protein_66 hypothetical protein -
P6H86_RS23850 (52092) 52092..52397 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
P6H86_RS23855 (52399) 52399..52617 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
P6H86_RS23860 (53209) 53209..53697 + 489 WP_011254646.1 hypothetical protein -
P6H86_RS23865 (53731) 53731..54864 - 1134 WP_001542067.1 DUF3800 domain-containing protein -
P6H86_RS23870 (55031) 55031..55804 - 774 WP_000905949.1 hypothetical protein -
P6H86_RS23875 (55817) 55817..56317 - 501 WP_000528931.1 HEPN family nuclease -
P6H86_RS23880 (56582) 56582..56812 + 231 WP_001261278.1 type II toxin-antitoxin system VapB family antitoxin -
P6H86_RS23885 (56809) 56809..57225 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid erm(42) / ant(3'')-Ia / sitABCD / fosA3 / blaTEM-1B / blaCTX-M-55 / aac(3)-IId / mph(A) iutA / iucD / iucC / iucB / iucA 1..159910 159910
- flank IS/Tn - - 50437..51636 1199


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T276001 WP_001159868.1 NZ_CP121158:c52397-52092 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT276001 WP_000813634.1 NZ_CP121158:c52617-52399 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References