Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1212005..1212623 Replicon chromosome
Accession NZ_CP121157
Organism Escherichia coli strain E89

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag P6H86_RS05850 Protein ID WP_001291435.1
Coordinates 1212005..1212223 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag P6H86_RS05855 Protein ID WP_000344800.1
Coordinates 1212249..1212623 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P6H86_RS05815 (1207294) 1207294..1207866 + 573 WP_000779831.1 YbaY family lipoprotein -
P6H86_RS05820 (1207897) 1207897..1208208 - 312 WP_000409911.1 MGMT family protein -
P6H86_RS05830 (1208587) 1208587..1208940 + 354 WP_000878140.1 DUF1428 domain-containing protein -
P6H86_RS05835 (1208982) 1208982..1210532 - 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
P6H86_RS05840 (1210696) 1210696..1211166 - 471 WP_000136192.1 YlaC family protein -
P6H86_RS05845 (1211282) 1211282..1211833 - 552 WP_000102564.1 maltose O-acetyltransferase -
P6H86_RS05850 (1212005) 1212005..1212223 - 219 WP_001291435.1 HHA domain-containing protein Toxin
P6H86_RS05855 (1212249) 1212249..1212623 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
P6H86_RS05860 (1213169) 1213169..1216318 - 3150 WP_066019159.1 efflux RND transporter permease AcrB -
P6H86_RS05865 (1216341) 1216341..1217534 - 1194 WP_066019158.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T275980 WP_001291435.1 NZ_CP121157:c1212223-1212005 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T275980 NZ_CP121157:c1212223-1212005 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT275980 WP_000344800.1 NZ_CP121157:c1212623-1212249 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT275980 NZ_CP121157:c1212623-1212249 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References