Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 41757..42400 Replicon plasmid pE67-1
Accession NZ_CP121156
Organism Escherichia coli strain E67

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag P6H85_RS24000 Protein ID WP_001044768.1
Coordinates 41757..42173 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag P6H85_RS24005 Protein ID WP_001261287.1
Coordinates 42170..42400 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P6H85_RS23985 (38256) 38256..38846 - 591 WP_000194575.1 hypothetical protein -
P6H85_RS23990 (38846) 38846..39103 - 258 WP_000343085.1 hypothetical protein -
P6H85_RS23995 (39457) 39457..41595 + 2139 WP_000350638.1 AAA family ATPase -
P6H85_RS24000 (41757) 41757..42173 - 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
P6H85_RS24005 (42170) 42170..42400 - 231 WP_001261287.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
P6H85_RS24010 (42696) 42696..42986 + 291 WP_000111771.1 hypothetical protein -
P6H85_RS24015 (42976) 42976..43875 + 900 WP_000963206.1 nucleotide-binding protein -
P6H85_RS24020 (43925) 43925..46150 - 2226 WP_000698737.1 P-loop NTPase fold protein -
P6H85_RS24025 (46152) 46152..47240 - 1089 WP_000952217.1 transcriptional repressor PifC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaCTX-M-27 / aadA2 / cmlA1 / ant(3'')-Ia / sul3 / aph(3')-Ia / tet(A) / mph(A) / dfrA14 - 1..124410 124410


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T275972 WP_001044768.1 NZ_CP121156:c42173-41757 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT275972 WP_001261287.1 NZ_CP121156:c42400-42170 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References