Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4705656..4706272 Replicon chromosome
Accession NZ_CP121155
Organism Escherichia coli strain E67

Toxin (Protein)


Gene name vapC Uniprot ID A0A176ZIX8
Locus tag P6H85_RS22695 Protein ID WP_001129490.1
Coordinates 4705656..4706030 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A1M0CY48
Locus tag P6H85_RS22700 Protein ID WP_001275523.1
Coordinates 4706030..4706272 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P6H85_RS22680 (4703159) 4703159..4704061 + 903 WP_000331377.1 formate dehydrogenase O subunit beta -
P6H85_RS22685 (4704058) 4704058..4704693 + 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
P6H85_RS22690 (4704690) 4704690..4705619 + 930 WP_000027720.1 formate dehydrogenase accessory protein FdhE -
P6H85_RS22695 (4705656) 4705656..4706030 - 375 WP_001129490.1 type II toxin-antitoxin system VapC family toxin Toxin
P6H85_RS22700 (4706030) 4706030..4706272 - 243 WP_001275523.1 CopG family transcriptional regulator Antitoxin
P6H85_RS22705 (4706492) 4706492..4706710 - 219 WP_001251290.1 CopG family transcriptional regulator -
P6H85_RS22710 (4707384) 4707384..4708298 - 915 WP_072649161.1 transposase -
P6H85_RS22715 (4708311) 4708311..4709198 - 888 Protein_4442 hypothetical protein -
P6H85_RS22720 (4709614) 4709614..4710555 - 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
P6H85_RS22725 (4710600) 4710600..4711037 - 438 WP_000560983.1 D-aminoacyl-tRNA deacylase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-115)

Antitoxin

Ribbon-helix-helix protein, CopG

(15-48)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13960.19 Da        Isoelectric Point: 7.0126

>T275971 WP_001129490.1 NZ_CP121155:c4706030-4705656 [Escherichia coli]
MAKRTALFDTNILIDLFSGRIEAKQALEAWPLQNAISLVTWMEVLVGAKKYDQEHRTRIAMSAFNVIGISQDIAERSVAL
RQEYRMKLPDAIILATAQIYGFELVTRNTRDFAGIAGVVTPYEL

Download         Length: 375 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9290.54 Da        Isoelectric Point: 4.3774

>AT275971 WP_001275523.1 NZ_CP121155:c4706272-4706030 [Escherichia coli]
MSAMAGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVDQYLENQSKTTISSAFGIWQGCEEDGVEYQRKLREEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A176ZIX8

Antitoxin

Source ID Structure
AlphaFold DB A0A1M0CY48

References