Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4179799..4180375 Replicon chromosome
Accession NZ_CP121155
Organism Escherichia coli strain E67

Toxin (Protein)


Gene name shpA Uniprot ID A0A2U2VE01
Locus tag P6H85_RS20255 Protein ID WP_001131965.1
Coordinates 4180088..4180375 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A2U2VE39
Locus tag P6H85_RS20250 Protein ID WP_000063158.1
Coordinates 4179799..4180101 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P6H85_RS20230 (4174907) 4174907..4176127 + 1221 WP_000873756.1 amino acid deaminase -
P6H85_RS20235 (4176395) 4176395..4178545 + 2151 WP_267657476.1 pyruvate/proton symporter BtsT -
P6H85_RS20240 (4178595) 4178595..4178798 + 204 WP_000467859.1 YbdD/YjiX family protein -
P6H85_RS20245 (4178809) 4178809..4179765 + 957 WP_001361361.1 GTPase -
P6H85_RS20250 (4179799) 4179799..4180101 - 303 WP_000063158.1 BrnA antitoxin family protein Antitoxin
P6H85_RS20255 (4180088) 4180088..4180375 - 288 WP_001131965.1 BrnT family toxin Toxin
P6H85_RS20260 (4180572) 4180572..4181738 + 1167 WP_000800823.1 restriction endonuclease -
P6H85_RS20265 (4181783) 4181783..4181956 + 174 Protein_3969 type I restriction-modification system subunit M N-terminal domain-containing protein -
P6H85_RS20270 (4181950) 4181950..4182618 + 669 Protein_3970 DUF3387 domain-containing protein -
P6H85_RS20275 (4182833) 4182833..4183171 + 339 WP_000132633.1 endoribonuclease SymE -
P6H85_RS20280 (4183259) 4183259..4183306 - 48 WP_229017048.1 hypothetical protein -
P6H85_RS20285 (4183554) 4183554..4184966 + 1413 WP_000199309.1 PLP-dependent aminotransferase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11223.71 Da        Isoelectric Point: 7.4021

>T275967 WP_001131965.1 NZ_CP121155:c4180375-4180088 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11537.99 Da        Isoelectric Point: 9.4646

>AT275967 WP_000063158.1 NZ_CP121155:c4180101-4179799 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDSDVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2U2VE01

Antitoxin

Source ID Structure
AlphaFold DB A0A2U2VE39

References