Detailed information of TA system TA027595

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 58466..59001
• Replicon: plasmid p12579_2
• Accession: NC_017657
• Organism: Escherichia coli O55:H7 str. RM12579

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: ECO55CA74_RS27315
• Protein ID: WP_001270421.1
• Coordinates: 58466..58753 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A1V0M724
• Locus tag: ECO55CA74_RS27320
• Protein ID: WP_001132893.1
• Coordinates: 58750..59001 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECO55CA74_RS27285	53869..54048	-	180	WP_000526857.1	hypothetical protein	-
ECO55CA74_RS27290	54251..54625	+	375	WP_001368887.1	hypothetical protein	-
ECO55CA74_RS31375	54936..55097	-	162	Protein_68	epoxyqueuosine reductase QueH	-
ECO55CA74_RS27730	55148..55288	+	141	Protein_69	serine/threonine protein kinase	-
ECO55CA74_RS27300	55412..56152	+	741	WP_001066926.1	tyrosine-type recombinase/integrase	-
ECO55CA74_RS27305	56436..57413	-	978	WP_000361616.1	RepB family plasmid replication initiator protein	-
ECO55CA74_RS29885	58059..58464	-	406	Protein_72	DDE-type integrase/transposase/recombinase	-
ECO55CA74_RS27315	58466..58753	-	288	WP_001270421.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ECO55CA74_RS27320	58750..59001	-	252	WP_001132893.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
ECO55CA74_RS27325	59390..60595	+	1206	WP_000817637.1	AAA family ATPase	-
ECO55CA74_RS27330	60592..61554	+	963	WP_000756329.1	ParB/RepB/Spo0J family partition protein	-
ECO55CA74_RS27335	61695..62706	-	1012	Protein_77	DUF4113 domain-containing protein	-
ECO55CA74_RS27340	62737..63153	+	417	Protein_78	DUF1281 domain-containing protein	-
ECO55CA74_RS30775	63157..63462	+	306	WP_032163448.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	stcE / exeE / exeG / nleA/espI	1..66078	66078

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11244.27 Da
• Isoelectric Point: 10.4703

>T27595 WP_001270421.1 NC_017657:c58753-58466 [Escherichia coli O55:H7 str. RM12579]
MRYQVKFREDALKEWQKLDKTIQQQFAKKLKKCCDNPHIPSAKLRGIKDCYKIKLRASGFRLVYQVIDEQLIIAVVAVGK
RERSDVYNLASERMR

Nucleotide

• Length: 288 bp

>T27595 NC_017657:c58753-58466 [Escherichia coli O55:H7 str. RM12579]
ATGAGGTATCAGGTAAAATTCAGGGAAGATGCGCTGAAAGAGTGGCAAAAACTGGACAAGACTATTCAGCAACAGTTTGC
GAAAAAGCTAAAAAAGTGCTGTGACAATCCGCATATTCCTTCCGCAAAACTGCGCGGGATAAAGGACTGCTACAAAATAA
AATTACGTGCGTCAGGTTTTCGCCTAGTCTATCAGGTGATTGACGAACAATTAATTATCGCTGTTGTAGCTGTGGGTAAG
CGTGAGCGCAGTGACGTTTATAATCTTGCCAGTGAAAGAATGAGATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9192.50 Da
• Isoelectric Point: 4.2108

>AT27595 WP_001132893.1 NC_017657:c59001-58750 [Escherichia coli O55:H7 str. RM12579]
MPNIILSDTSASVSELKKNPMATVSAGDGFPIAILNRNQPAFYCVPAELYEKMLDALDDQELVKLVAERSNQPLHDVDLD
KYL

Nucleotide

• Length: 252 bp

>AT27595 NC_017657:c59001-58750 [Escherichia coli O55:H7 str. RM12579]
ATGCCTAACATTATTCTCAGTGATACAAGCGCCAGTGTCAGCGAGCTGAAGAAAAATCCGATGGCGACAGTCAGCGCCGG
TGATGGTTTCCCGATCGCTATCCTGAACCGTAATCAGCCTGCTTTCTACTGTGTACCCGCAGAGCTGTACGAAAAGATGC
TCGATGCCCTGGACGATCAGGAGCTGGTTAAACTGGTAGCCGAACGCAGCAACCAACCGCTGCATGATGTAGACCTAGAT
AAATACCTATGA

Structures

Antitoxin

• AlphaFold DB: A0A1V0M724