Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 13453..14096 Replicon plasmid pE57-1
Accession NZ_CP121154
Organism Escherichia coli strain E57

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag P6H84_RS23985 Protein ID WP_001044768.1
Coordinates 13680..14096 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag P6H84_RS23980 Protein ID WP_001261287.1
Coordinates 13453..13683 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P6H84_RS23960 (8613) 8613..9701 + 1089 WP_000952217.1 transcriptional repressor PifC -
P6H84_RS23965 (9703) 9703..11928 + 2226 WP_000698737.1 P-loop NTPase fold protein -
P6H84_RS23970 (11978) 11978..12877 - 900 WP_000963206.1 nucleotide-binding protein -
P6H84_RS23975 (12867) 12867..13157 - 291 WP_000111771.1 hypothetical protein -
P6H84_RS23980 (13453) 13453..13683 + 231 WP_001261287.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
P6H84_RS23985 (13680) 13680..14096 + 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
P6H84_RS23990 (14258) 14258..16396 - 2139 WP_000350638.1 AAA family ATPase -
P6H84_RS23995 (16750) 16750..17007 + 258 WP_000343085.1 hypothetical protein -
P6H84_RS24000 (17007) 17007..17597 + 591 WP_000194575.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(A) / aph(3')-Ia / sul3 / ant(3'')-Ia / cmlA1 / aadA2 / blaCTX-M-27 / dfrA14 / mph(A) - 1..124410 124410


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T275946 WP_001044768.1 NZ_CP121154:13680-14096 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT275946 WP_001261287.1 NZ_CP121154:13453-13683 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References