Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4725157..4725773 Replicon chromosome
Accession NZ_CP121153
Organism Escherichia coli strain E57

Toxin (Protein)


Gene name vapC Uniprot ID A0A176ZIX8
Locus tag P6H84_RS22870 Protein ID WP_001129490.1
Coordinates 4725157..4725531 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A1M0CY48
Locus tag P6H84_RS22875 Protein ID WP_001275523.1
Coordinates 4725531..4725773 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P6H84_RS22855 (4722660) 4722660..4723562 + 903 WP_000331377.1 formate dehydrogenase O subunit beta -
P6H84_RS22860 (4723559) 4723559..4724194 + 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
P6H84_RS22865 (4724191) 4724191..4725120 + 930 WP_000027720.1 formate dehydrogenase accessory protein FdhE -
P6H84_RS22870 (4725157) 4725157..4725531 - 375 WP_001129490.1 type II toxin-antitoxin system VapC family toxin Toxin
P6H84_RS22875 (4725531) 4725531..4725773 - 243 WP_001275523.1 CopG family transcriptional regulator Antitoxin
P6H84_RS22880 (4725993) 4725993..4726211 - 219 WP_001251290.1 CopG family transcriptional regulator -
P6H84_RS22885 (4726885) 4726885..4727799 - 915 WP_072649161.1 transposase -
P6H84_RS22890 (4727812) 4727812..4728699 - 888 Protein_4476 hypothetical protein -
P6H84_RS22895 (4729115) 4729115..4730056 - 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
P6H84_RS22900 (4730101) 4730101..4730538 - 438 WP_000560983.1 D-aminoacyl-tRNA deacylase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-115)

Antitoxin

Ribbon-helix-helix protein, CopG

(15-48)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13960.19 Da        Isoelectric Point: 7.0126

>T275945 WP_001129490.1 NZ_CP121153:c4725531-4725157 [Escherichia coli]
MAKRTALFDTNILIDLFSGRIEAKQALEAWPLQNAISLVTWMEVLVGAKKYDQEHRTRIAMSAFNVIGISQDIAERSVAL
RQEYRMKLPDAIILATAQIYGFELVTRNTRDFAGIAGVVTPYEL

Download         Length: 375 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9290.54 Da        Isoelectric Point: 4.3774

>AT275945 WP_001275523.1 NZ_CP121153:c4725773-4725531 [Escherichia coli]
MSAMAGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVDQYLENQSKTTISSAFGIWQGCEEDGVEYQRKLREEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A176ZIX8

Antitoxin

Source ID Structure
AlphaFold DB A0A1M0CY48

References